360 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Sterilization: Sterilize at 120° for 20 



minutes. 

 Use: General culture medium. 

 Variants: Kahn prepared the medium as 



follows: 



(1) Free fresh cod fish from bone and skin, 

 chop into small pieces and grind in a 

 mortar. 



(2) Add 800.0 cc. tap water for each pound 

 of fish and boil gently for 45 minutes. 



(3) Adjust the reaction to pH = 7.2. 

 Prior to testing mix the fish muscle 

 and broth so that one verticle inch of 

 the tissue is present in each tube. 



(4) Autoclave at 15 pounds pressure for 

 20 minutes. 



(5) The tubes are sealed with a vaseline 

 cap. 



References: Harde and Hauser (1919 p. 

 1259), Kahn (1922 p. 174). 



1236. Stoklasa's Bone Meal Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. CaS04 1.0 g. 



3. MgCl2 0.5 g. 



4. FeS04 0.1 g. 



5. Bone meal 10.0 g. 



Preparation : 



(1) Dissolve 2, 3, and 4 in a liter of 

 distilled water. 



(2) Place 10.0 g. of finely ground bone 

 meal into a flask. (Bone contains 

 19.0% H3PO4, 5.26% Nitrogen and 

 1.5% fat). 



(3) Add 100.0 cc. of (1) and 800.0 cc. of 

 water to (2). 



Sterilization: Method not given. 

 Use: To study bone decomposition. 

 Variants: The author used 1.0 g. K2SO4 



instead of 1.0 g.CaS04. 

 Reference: Stoklasa (1900 p. 529), (1911 



p. 472). 



1237. Remy and Rosing's Horn Shavings 

 Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Horn Shavings 37.5 g. 



Preparation: 



(1) Dry finely divided "Hornspahne." 



(2) Add 0.75 g. of (1) to 20.0 cc. sterile 



water. 



(3) Distribute into small Erlenmeyer 

 flasks. 

 Sterilization: Sterilize in streaming steam 



by means of the fractional method. 

 Use: To study decomposition of organic 



N materials by soil forms. 

 Reference: Remy and Rosing (1911 p. 39). 



1238. Cunningham's Hornmeal Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Hornmeal (1.0%) 10.0 g. 



3. K2HPO4 (0.05%) 0.5 g. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Distribute in 10.0 cc. lots in 50.0 cc. 

 flasks or tubes. 



Sterilization: Sterilize at 1.5 atmospheres 



pressure. 

 Use: To study ammonification by soil and 



manure forms, Bac. mycoides, Bad. 



ftuorescens, Bad. vulgar e, Bad. coli, 



Sarcina lutea, Bac. putrificus. 

 Reference: Cunningham (1924 p. 149). 



1239. Migula's Meat Medium 



Constituents: 



1. Water. 



2. Meat. 

 Preparation : 



(1) Chop lean beef into small pieces. 



(2) Add (1) to Erlenmeyer flasks until a 

 layer about a finger deep is formed. 



(3) Add water just to cover the meat 

 layer. 



Sterilization: Sterilize the flasks for 30 

 minutes on each of 3 successive days. 



Use: Differentiation of colon and typhoid 

 bacilli. Author reported that the colon 

 bacilli produced a fetid odor while ty- 

 phoid bacilli did not. Holman cultivated 

 anaerobes, and Besson Vibrion septique 

 in similar media. 



Variants : 



(a) Holman cultivated anaerobes from 

 war wounds on a medium prepared as 

 follows: 



(1) Grind fresh finely chopped fat and 

 fiber free beef muscle in a mortar. 



(2) Mix (1) with equal parts of water. 



(3) Heat slowly to boiling with con- 

 stant stirring. 



(4) Neutralize to rosalic acid or phenol- 

 phthalein. 



