364 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Added nutrients: The authors used any 

 desired carbohydrate, alcohol, etc. 



Reference: Hollande and Fumey (1917 

 p. 836). 



1247. Waksman's Basal Albumin Solution 



Constituents: 



1. Water 1000.0 cc 



2. Glycerol 30.0 g 



3. K2HPO4 1.0 g 



4. KCl 0.5 g 



5. MgSOi 0.5 g 



6. FeS04 0.01 g 



7. Egg albumin (powdered).. 5.0 g 

 Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and one of the 

 added nutrients in 1. 



(2) Dissolve powdered egg albumin in 

 N/10 NaOH, and add to (1). 



(3) Tube in 10-12 cc. lots. 

 Sterilization: Sterilize at 15 pounds for 15 



minutes. 

 Use: To study metabolism of actinomy- 



cetes. 

 Added nutrients: The author added 2.0 g. 

 of one of the following : 



NaNOs (NH 4)2804 



NaN02 (NH4)2C03 



Reference: Waksman (1920 p. 3). 



1248. Kent's Glycerol Albumin Solution 



Constituents: 



1. Egg albumin 1000.0 cc. 



2. Glycerol (33.0%) 333.0 g. 



Preparation: 



(1) Add 33.0% or less glycerol to egg 

 albumin. 



Sterilization: Not specified. 



Use: Cultivation of virus vaccinia. The 

 author reported that the diplo-bacillus 

 vacciniae grew in a cotton-like mass. 

 The glycerol content tended to reduce the 

 growth of staphylococci and other con- 

 taminating forms. The medium may be 

 coagulated with heat. (Details not 

 given.) 



Reference: Kent (1898 p. 1393). 



1249. Barthel's Albumin Solution 



Constituents: 



1. Egg albumin. 

 Preparation: 



(1) Prepare a 2.0% Na-COs solution. 



(2) Add 8.0 cc. of (1) to Gruber tubes 

 containing sterile egg albumin. 



Sterilization: Sterilization of Na^COs solu- 

 tion not given. Sterilize the egg albumin 

 at 125 to 130°C. 



Use: Cultivation of anaerobic bacteria 

 found in milk. The author added 0.1 cc. 

 to 8.0 cc. of Na2C03 solution, and then 

 added the sterile egg albumin. The air 

 was removed. 



Reference: Barthel (1910 p. 6). 



1250. Bainbridge's Albumin Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Egg albumin 1.0 to 5.0 g. 



3. NaCl 5.0 g. 



4. Na2S04 1.0 to 2.5 g. 



5. CaClz trace 



6. Potassium phosphate.. trace 

 Preparation : 



(1) Dissolve 2 (proteins used were egg 

 albumin, serum protein and alkali- 

 albumin) , 3, 4, 5 and 6 in 1. 



(2) Add N/100 H2SO4 until the medium is 

 very faintly alkaline to extremely 

 sensitive red litmus paper. 



(3) After sterilization, distribute in 5.0 

 cc. quantities. 



Sterilization: Sterilize by passing thru a 

 Berkefeld filter. 



Use: To study multiplication of bacteria, 

 in pure protein solutions. The author 

 reported very slight growth in pure pro- 

 tein media with any of the organisms 

 used. 



Reference: Bainbridge (1911 p. 343). 



1251. Castellani's Lactose Albimiin Solution 



(Stitt) 



Constituents: 



1. Water 1000.0 cc. 



2. Lactose (1.0%,) 10.0 g. 



3. Egg albumin (10.0%) 100.0 g. 



Preparation: 



(1) Dissolve 2 and 3 in 1. 

 Sterilization: Method not given. 

 Use : Cultivation of intestinal protozoa and 



amoeba. Used also to replace the water 



of condensation in agar slants. 

 Reference: Stitt (1923 p. 51). 



1252. Hogue's Ovomucoid Medium (Hegner 



and Becker) 



Constituents: 



1. Water 600.0 cc. 



