CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



365 



2. NaCl (0.7%) 4.2 g. 



3. Egg white 

 Preparation : 



(1) Thoroughly shake up the whites of 

 six eggs with glass beads. 



(2) Add (1) to 600.0 cc. of 0.7% saline 

 solution. 



(3) Cook for 20 to 30 minutes over a boil- 

 ing water bath, agitating constantly. 



(4) Strain thru cheese cloth. 



(5) Filter thru cotton using a suction 

 pump. 



(6) Tube in 5.0 cc. lots. 

 Sterilization.: Sterilize at 15 pounds pres- 

 sure for 20 minutes. 



Use: Cultivation of Trichomonas hominis 

 and Chilomastix mesnili and other in- 

 testinal flagellates. 



Reference: Hegner and Becker (1922 p. 18). 



1253. Hiss' Basal Serum Solution 



Constituents: 



1. Water 200.0 cc. 



2. Serum 100.0 cc. 



Preparation: 



(1) Mix two parts water with one part 

 serum and heat to 100°C. for some 

 minutes. 



(2) Dissolve one of the added nutrients 

 in (1). 



Sterilization: Sterilize at 68°C. for one hour 

 on six consecutive days. 



Use: To study fermentation by highly 

 parasitic forms. Hiss used pneumococci 

 and streptococci and reported that 

 pneumococci induced coagulum with 

 glycogen in 24 hours. Streptococci no 

 coagulum. Both organisms produced co- 

 agulum with other materials. 



Added nutrients and variants: 



(a) The author added 3.0 g. of one of the 

 following: 



glycogen galactose ; 



glucose maltose 



lactose sucrose 



(b) The author mixed one part ox serum 

 with two parts distilled water and 

 added 0.1% normal NaOH. Pneu- 

 mococci caused coagulation after 

 several days in this medium while 

 streptococci did not. 



(c) Hiss and Russell diluted 100.0 cc. of 

 beef serum with 200.0 or 300.0 cc. of 

 distilled water, boiled, added 1.0% 



of any desired carbohydrate, alcohol, 

 etc., added 1.0% of a 5.0% solution of 

 Merck's highly purified litmus solu- 

 tion, tubed and sterilized for 10 

 minutes on each of 3 successive days 

 in streaming steam. 



(d) Elser and Huntoon prepared the 

 medium as follows: 



(1) Mix one part sheep serum with two 

 parts distilled water. 



(2) Add from 3.0 to 4.5 cc. of a watery 

 solution of Merck's highly sen- 

 sitized litmus. 



(3) Sterilize (method not given). 



(4) Prepare a 10.0% solution of one of 

 the following in distilled water: 



glucose mannitol 



galactose dulcitol 



levulose inulin 



lactose dextrin 



maltose sucrose 



(5) Sterilize (4) at 100°C. for 10 

 minutes. 



(6) Mix (5) and (3) so that the added 

 nutrient be present in 1.0% con- 

 centration. 



(7) Tube in sterile tubes. 



(8) Incubate for 3 days to detect acci- 

 dental contamination. 



(e) Distaso pepared the medium as 

 follows: 



(1) Dilute one volume of beef or sheep 

 serum with three volumes of water. 



(2) Sterilize at 120° for 15 minutes. 



(3) Dissolve 1.0% of the desired car- 

 bohydrate, alcohol, etc., in (2). 



(4) Sterilize (method not given). 



(f) Krumwiede, Pratt and Kahn gave the 

 following medium for the cultivation 

 of the paratyphoid enteritidis group: 



(1) To 400.0 cc. sterile distilled water 

 add 100.0 cc. of sterile horse serum, 

 5.0 cc. of Andrades indicator and 

 2.5 cc. of a sterile 25.0% solution of 

 glucose, or any other desired car- 

 bohydrate, alcohol etc., in distilled 

 water (final concentration of glu- 

 cose 0.1%o). 



(2) Tube to a depth of 4.0 cc. and steam 

 sterilize intermittently on two suc- 

 cessive days for 10 minutes. The 

 medium may be prepared from non- 

 sterile materials and sterilized 

 intermittently on 3 successive days. 



