CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



367 



successive days. Sterilize the in- 

 ulin solution in the autoclave. 



(5) Add sufficient of (4) to (3) to give a 

 1.0% concentration of the added 

 nutrient under aseptic conditions. 

 Generally 5.0% glycerol is used 

 instead of 1.0%. 



(6) In routine work with glucose, 

 lactose, sucrose, mannitol, and 

 dulcitol it is generally sufficient to 

 add the sugar to the medium and 

 sterilize in the Arnold for 30 min- 

 utes on each of 3 successive days. 



References: Hiss (1901-05 pp. 325, 327), 

 Hiss and Russel (1903 pp. 289, 295) Elser 

 and Huntoon (1909 p. 404), Distaso (1916 

 p. 600), Roddy (1917 p. 42), Baeslack and 

 Keane (1920 p. 392), Giltner (1921 p. 383), 

 Krumwiede, Pratt and Kahn (1916-17 p. 

 357), Abbott (1921 p. 143), Harvey (1921- 

 22 p. 78), Gildemeister (1921-22 p. 76), 

 Klimmer (1923 p. 211), Stitt (1923 p. 

 36), Park, Williams and Krumwiede 

 (1924 p. 123). 



1254. Leuch's Basal Serum Solution 

 (Klimmer) 



Constituents: 



1. Water 800.0 cc. 



2. Serum 180.0 cc. 



3. NaCl (0.5%) 5.0 g. 



4. NaOH (15.0% soln.) 20.0 cc. 



Preparation : 



(1) Mix 9 parts serum with 1 part of a 

 15.0% NaOH solution, and incubate 

 at37°C. for 2 days. 



(2) Make only slightly alkaline to litmus 

 by the addition of 25% HCl. 



(3) Dilute one part (2) with 4 parts water 

 and add 0.57o NaCl. 



(4) After sterilization, filter. 



(5) Dissolve one of the added nutrients in 

 50.0 cc. of Kubel and Tiemann's 

 litmus solution that has been boiled 

 for 15 minutes. 



(6) Heat (5) in a boiling water bath for 

 6 to 8 minutes. 



(7) Filter (6) until clear. 



(8) Add (7) to 1000.0 cc. of (4). 



(9) Tube. 



Sterilization: Method of sterilization of 

 (4) not specified. Sterilize the medium 

 following distribution into tubes on each 

 of 3 successive days for 10 minutes. 



Use: To study fermentation. 



Added nutrients : Klimmer added one of the 



following: 



glucose 10.0 g. 



lactose 10.0 g. 



maltose 20.0 g. 



sucrose 20.0 g. 



mannitol 20.0 g. 



Reference: Klimmer (1923 p. 211). 



1255. Lorrain-Smith's Alkaline Serum 

 Solution (Heinemann) 



Constituents: 



1. Serum 1000.0 cc. 



2. NaOH (10.0% soln.) 



(1.0 to 1.5%) 10.0 to 15.0 cc. 



Preparation : 



(1) Add 1.0 to 1.5% of a 10.0% solution 

 of sodium hydrate to blood serum. 



(2) Tube. 



Sterilization: Sterilize in the Arnold or 



Koch's serum inspissator. 

 Use: General culture medium. 

 Reference: Heinemann (1905 p. 128). 



1256. Klein's Basal Alkaline Serum 

 Solution (Leuch) 



Constituents: 



1. Water 400.0 cc. 



2. Serum 90.0 cc. 



3. NaOH (15.0%) 10.0 cc. 



4. Litmus 



5. NaCl (0.5%) 2.5 g. 



Preparation : 



(1) Mix nine parts serum (horse) with 

 one part 15.0% NaOH. 



(2) Incubate at 37°C. for two days. 



(3) Make only slightly alkaline to litmus 

 by the addition of 25.0% HCl. 



(4) Dilute one part serum with four 

 parts water and add 0.5% NaCl. 



(5) Sterilize in the autoclave. 



(6) Allow to cool and filter. 



(7) Boil Kubel and Tiemann's litmus 

 solution for 15 minutes. 



(8) Dissolve one of the added nutrients 

 in (7) by boiling 6 to 8 minutes. 



(9) Mix (8) and (6) in the ratio of 1:20, 

 while both are warm. 



(10) Tube. 

 Sterilization: Sterilize on each of three 



successive days for ten minutes each day. 

 Use: Detection of typhoid-paratyphoid 



and cholera organisms. 



