CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



369 



(c) Wang tubed serum (ox) in 2.5 cc. 

 quantities and heated at 56°C. for 

 3 or more successive days until the 

 serum became syrupy. The medium 

 was used in the diagnosis of diph- 

 theria. 



(d) Harvey cultivated spirochaetes in 

 sterile rabbit serum heated 30 min- 

 utes at 58° to 60°C. covered with a 

 layer of sterile paraffin oil. 



References: Davis (1903 p. 405), Longcope 

 (1905 p. 627), Schereschewsky (1909 p. 

 35), Wang (1919 p. 233), Harvey (1921- 

 22 p. 80). 



1258. Marmier's Serum Solution. 



Constituents : 



1. Water 100.0 cc. 



2. Serum (beef) 100.0 cc. 



Preparation : 



(1) Mix equal parts of beef serum and 

 water. 



(2) Make slightly alkaline by the addition 

 of soda. 



Sterilization: Sterilize at 115° in the co- 



agulator (time not specified). 

 Use: Anthrax toxin production by anthrax 



bacilli. 

 Reference: Marmier (1895 p. 569). 



1259. Boeck's Glucose Serxim Solution. 



Constituents: 



1. Distilled water 1000.0 cc. 



2. NaHC03 0.2 g. 



3. KCl 0.42 g. 



4. CaCh 0.24 g. 



5. NaCl 9.0 g. 



6. Glucose 2.5 g. 



7. Serum (human, sheep or 



horse) 250.0 cc. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Mix aseptically one part sterile serum 

 (preferably human) with 4 parts 

 sterile (1). The usual reaction of the 

 mixture is 0.2% alkaline to phenol- 

 phthalein. 



(3) Tube in 5.0 cc. lots in sterile tubes. 



(4) Incubate at 37° over night to test 

 sterility. 



Sterilization: Method of sterilization of 



(1) not given. 

 Use: Cultivation of Chilomastix mesnili, 



a parasite in the small intestine of man. 

 Reference: Boeck (1921 p. 159). 



1260. Sinton's Glucose Serum Solution 



Constituents: 



1. Serum, horse 1000.0 cc. 



2. Glucose (50.0% soln.) 15.0 cc. 



Preparation : 



(1) Add 1.5 cc. of a 50.0%, sterile glucose 

 solution to each 100.0 cc. of asepti- 

 cally collected horse serum. 

 Sterilization : Not specified. 

 Use: Cultivation of Spirochaeta carteri 



causing Indian relapsing fever. 

 Reference: Sinton (1923-24 p. 826). 



1261. Toyoda's Glucose Serum Solution 

 Constituents: 



1. Serum, dog 1000.0 cc. 



2. Glucose 10-0 g. 



3. Sodium citrate solution 



4. Physiological salt solution 

 Preparation: 



(1) To each 10.0 cc. of healthy young dog 

 serum add 0.2 cc. of a 50.0%, sterile 

 glucose solution and 0.3 cc. of a 

 2.0% sterile sodium citrate in 0.85% 

 NaCl solution. 



(2) Pipette the serum in small narrow 

 test tubes 5 cm. high and inactivate 

 the serum at 45°C. for one hour. 



Sterilization: Not specified. 



Use: Cultivation of Babesia cannis 



Reference: Toyoda (1913 p. 76). 



1262. Marbais' Lactose Serum Solution 



Constituents: 



1. Distilled water 300.0 cc. 



2. Serum 100.0 cc. 



3. Lactose 8.0 g. 



4. Litmus 

 Preparation : 



(1) Add one volume of clear serum (man 

 or animal, preferably horse or sheep) 

 to three volumes of neutral distilled 

 water. 



(2) Add sufficient lactose to make 2.0 g. 

 lactose per 100.0 cc. of mixture. 



(3) Mix well and filter thru paper. 



(4) Tube in 7 or 8.0 cc. lots. 



(5) Add 0.1 cc. of a sterile litmus solution 

 prepared according to Besson (refer- 

 ence or method not given) to each 

 sterile tube. 



(6) Incubate for 24 hours to test sterility. 

 Sterilization: Sterilize (4) at 120°C. for 15 



minutes. 

 Use: Differentiation of colon-typhoid ba- 



