370 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



cilli. Author reported that typhoid 

 cause coagulation after 24 hours, color 

 was blue-violet. Paratyphoid A clouded 

 the medium, and the color was lilac. 

 Paratyphoid B gave a red color after 5 

 or 6 hours incubation, then changed to 

 original color. B. coli coagulated and 

 decolorized the medium. 

 Reference: Marbais (1918 p. 602). 



1263. Hiss' Inulin Serum Solution 



Constituents: 



1. Distilled water 200.0 cc. 



2. Beef serum 100.0 cc. 



3. Inulin (pure) (1.0%) 3.0 g. 



4. Litmus (5.0% soln.) 3.0 cc. 



Preparation : 



(1) Mi.x two parts distilled water with one 

 part fresh clear beef serum. 



(2) Add 1.0% pure inulin, and 3.0 cc. of 

 5.0% solution Merck's highly purified 

 litmus. 



Sterilization: Sterilize at 100°C. for 10 

 minutes (if inulin contains heat resisting 

 spores, autoclave at 10 to 15 pounds 

 pressure for 15 minutes.) 



Use: Differentiation of pneumococci and 

 streptococci. Author reported that 

 pneumococci. cultures coagulated the 

 serum, streptococci did not. 



Variants : 



(a) Hiss omitted the litmus. 



(b) Hiss prepared a similar medium as 

 follows: 



(1) Add 4.0 g. powdered starch to 400.0 

 cc. water, and boil for 30 minutes. 

 Allow to stand over night. 



(2) Obtain, next morning, the clear 

 fluid by pipetting off the water 

 from the starch particles. 



(3) Add one part serum to two parts of 

 the supernatant fluid from (2). 



(4) Sterilize at 68°C. for 1 hour on 6 

 consecutive days. 



(c) Hiss heated the beef serum and water 

 at 100°C. for several minutes and 

 then added the inulin and litmus. 



(d) Harvey prepared the medium as 

 follows: 



(1) Mix 1 part ox serum with 3 parts 

 water. 



(2) Steam 15 minutes. 



(3) Add sufficient litmus solution to 

 give a blue color. 



(4) Prepare a 10.0% solution of inulin. 



(5) Sterilize (4) at 15 pounds pressure 

 for 15 minutes. 



(6) Mix 1 part (5) with 10 parts (3). 



(7) Sterilize on each of 3 successive 

 days for 20 minutes at 100°C. 



Reference: Hiss (1901-05 pp. 324, 330), 

 Harvey (1921-22 p. 112). 



1264. Legroux's Formol Serum Solution 

 Constituents: 



1. Distilled water 1200.0 cc. 



2. Serum (beef or horse) 600.0 cc. 



3. Formol 1.0 cc. 



Preparation: 



(1) Mix 600.0 cc. of beef or horse serum 

 with 1.0 cc. of commercial formol. 



(2) Dilute (1) with 1200.0 cc. distilled 

 water. 



(3) Mix well. 



(4) The reaction is acid to methyl red. 



(5) Distribute in tubes or flasks. 

 Sterilization: Sterilize in the autoclave at 



112 to 115° for varying lengths of time 

 depending on the size of the container. 



Use: Enrichment of meningococci. The 

 author reported that the formol kept the 

 medium from coagulating during steriliz- 

 ation. It also aided in giving a clear 

 medium. 



Reference: Legroux (1920 p. 466). 



1265. Marzinowsky's Citrated Blood 

 Solution 

 Constituents: 



1. Water 100.0 cc. 



2. Sodium citrate (10.0%) 10.0 g. 



3. Blood, horse 

 Preparation: 



(1) Prepare a 10.0% watery solution of 

 sodium citrate. 



(2) Distribute in 1.5 to 2.0 cc. lots in test 

 tubes. 



(3) Add 10.0 cc. of horse blood to each 

 tube of sterile (2). The blood is 

 taken directly from a vein. 



Sterilization: Sterilize (2); method not 



given. 

 Use: Cultivation of Piroplasma equi and 



other piroplasma. 

 Reference: Marzinowsky (1908-09 p. 419). 



1266. Griffith's Citrated Blood Solution 

 Constituents : 



1. Blood, citrated (horse, beef, 

 rabbit) 100.0 cc. 



2. Physiological salt solution... 200.0 cc. 



