CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



371 



Preparation : 



(1) Mix 1 part citrated blood (horse, beef 

 or rabbit) with 2 parts physiological 

 saline. 



(2) Heat until semi-gelatinous. 

 Sterilization: Not specified. 



Use: Cultivation of Spirochaeta ictero- 

 haernorrhagiae . The author inoculated 

 the medium with infected heart blood or 

 tissue fragment, and covered the medium 

 with a thin layer of paraffin oil. 



Reference: Griffith (1919-20 p. 61). 



1267, Besson's Citrated Blood 



Constituents: 



1. Water 1000.0 cc. 



2. NaCl 8.0 g. 



3. Sodium citrate 15.0 g. 



4. Blood 

 Preparation : 



(1) Dissolve 8.0 g. NaCl and 15.0 g. of 

 sodium citrate in 1000.0 cc. of water. 



(2) Mix 20.0 cc. of sterile (1) with 80.0 cc. 

 of blood obtained under aseptic 

 conditions. 



Sterilization: Sterilize (1); method not 



given 

 Reference: Besson (1920 p. 34). 



1268. Rogers' Citrated Blood (Stitt) 



Constituents: 



1. Blood. 



2. Sodium citrate (10.0%). 



3. Citric acid. 

 Preparation : 



(1) Place 1.0 or 2.0 cc. of a sterile 10.0% 

 sodium citrate slightly acidified with 

 citric acid into the barrel of a syringe 

 and aspirate splenic blood directly 

 into it. 



Sterilization: Not specified. 



Use: Cultivation of Leishmania. 



Reference: Stitt (1923 p. 52). 



1269. Buchner's Blood Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Blood 100.0 cc. 



3. Sucrose (10.0%) 100.0 g. 



Preparation : 



(1) Prepare a 10.0% solution of cane 

 sugar. (Reaction may be slightly 

 alkaline.) 



(2) Add 0.1 volume of blood to sterile (1). 



Sterilization: Sterilize (1) by heating at 

 55°C. 



Use : To show influence of sugar on develop- 

 ment of anthrax and other organisms. 

 Author reported that 10.0% sugar in- 

 creased the bacterial count. 



Variants: The author omitted the sucrose 

 or used 40.0% instead of 10.0%. 



Reference: Buchner (1890 p. 67). 



1270. Davis' Blood Medium 



Constituents : 



1. Blood, human. 

 Preparation: 



(1) Prick the extensor surface of the 

 thumb just back of the base of the 

 nail, the skin having been previously 

 cleansed with soap and water and 

 alcohol. 



(2) Applying a tourniquet at the base of 

 the thumb just tight enough to 

 obstruct the venous flow, a consider- 

 able quantity of blood may be 

 obtained. 



(3) The blood is drawn up by capillary 

 attraction into a small glass tube, 

 one end of which has been drawn out 

 to a very fine caliber over the Bunsen 

 flame, and the whole tube sterilized 

 by the same means. 



(4) When about 0.2 cc. of blood has 

 flowed into the tube the capillary end 

 may be sealed in the flame, and the 

 other end plugged with cotton, giving 

 a miniature culture tube. 



(5) The medium is nearly always sterile 

 when obtained in the manner in- 

 dicated. 



Sterilization: Given in the preparation. 



Use: Cultivation of Ducrey bacillus (chan- 

 croid bacillus), pneumococci and strep- 

 tococci. 



Variants: Stitt reported that pneumococci 

 and streptococci maintained their viru- 

 lence in a medium prepared as follows: 



(1) Obtain rabbit or human blood under 

 aseptic conditions and preserve 

 (whole blood) in small test tubes. 



(2) Heat for 30 minutes at 56°C. to 

 inactivate the blood. 



References: Davis (1903 p. 405), Stitt (1923 

 p. 44). 



