372 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



1271. Klimenko's Laked Blood Solution 



Constituents: 



1. Distilled water 900.0 cc. 



2. Blood 10.0 cc. 



Preparation: 



(1) Mix 10 parts blood and 90 parts 

 sterile distilled water. 



(2) Tube. 

 Sterilization: Not specified. 



Use: Cultivation of whooping cough bacil- 

 lus. Author reported that the organisms 

 grew first on the surface of the medium, 

 then on the bottom; after several days the 

 whole tube became turbid and was finally 

 colored dark brown. 



Reference: Klimenko (1909 p. 312). 



1272. Wurtz and Sappington's Diluted Blood 



Solution 

 Constituents: 



1. Water 18.0 cc. 



2. Blood 12.0 cc. 



Preparation : 



(1) Rub the skin at the bend of the elbow 

 with a pledget of cotton soaked in 

 alcohol. 



(2) Take the blood with a large sized 

 Wassermann needle kept dry — steril- 

 ized in a test tube. 



(3) Distend the vein by a knotted piece 

 of rubber tubing above, and the 

 patients clinched fist below. 



(4) Plunge the needle into the vein and 

 allow about 10.0 cc. of blood to run 

 into a dry sterile test tube. This 

 washes out the needle and lessens the 

 chance of contamination. 



(5) Run about 12.0 cc. of the blood then 

 into a tube containing 18.0 cc. of 

 sterile water. 



Sterilization: Sterilization given under 

 preparation. 



Use: Blood culture. Author reported that 

 this method was especially good in case 

 of pneumococcic and streptococcic in- 

 fections. 



Reference: Wurtz and Sappington (1918 

 p. 373). 



1273. Bass, Foster and Johns' Glucose 

 Blood Solution 

 Constituents: 



1. Blood (malarial) lOO.O cc. 



2. Glucose 0.5 g. 



Preparation : 



(1) Draw the blood from the patients 

 vein at the bend of the elbow. 



(2) Add 0.1 cc. of 50.0% dextrose solution 

 for every 10.0 cc. of blood drawn. 



(3) Defibrinate the blood by gently 

 whipping or stirring with a glass rod. 

 Avoid air bubbles. 



(4) After defibrination, plug the tube 

 with a fresh sterile plug. 



(5) The column of blood must be from 

 one to two inches thick. This gives a 

 column of serum Ho 1 inch thick. 



Sterilization: Not specified. 



Use: Cultivation of malarial plasmodia. 



Variants : 



(a) Harvey prepared the medium as 

 follows: 



(1) Add 15.0 cc. aspirated malarial 

 blood to a centrifuge tube con- 

 taining 0.1 cc. 50.0% glucose. 



(2) Defibrinate the blood-glucose mix- 

 ture with a glass rod. 



(3) Centrifuge. 



(4) Observe the development of para- 

 sites at 41 °C. under anaerobic 

 conditions. 



(b) Stitt gave the following method of 

 preparation: 



(1) Place 0.1 cc. of a 50.0% glucose 

 solution in a centrifuge tube. 



(2) Add from 10.0 to 20.0 cc. of malarial 

 patients blood to each tube of (1). 



(3) Defibrinate the blood by extending 

 a piece of glass rod or piece of 

 tubing into the bottom of the cen- 

 trifuge tube. 



(4) Centrifuge. 



(5) There should be at least one inch of 

 serum above the cell sediment. 



(c) Park, Williams and Krumwiede used 

 the following method : 



(1) Defibrinate carefully 10.0 cc. of 

 blood taken from a malarial 

 patient. 



(2) Place in small test tubes in 1.0 cc. 

 amounts. 



(3) One per cent of a 50 per cent 

 solution of dextrose is added to each 

 small test tube before adding the 

 blood. 



(4) The red corpuscles settle so that a 

 0.5 cm. layer of serum is left above 

 them. 



