CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



373 



References: Bass, Foster and Johns (1912 

 p. 570), Harvey (1921-22 p. 72), Stitt 

 (1923 p. 53), Park, Williams and Krum- 

 wiede (1924 p. 133). 



1274. Besson's Defibrinated Blood 



Constituents: 



1. Blood. 

 Preparation: 



(1) Collect blood under aseptic condi- 

 tions in a sterile flask containing glass 

 beads. 



(2) Shake thoroly for 10 minutes. 



(3) Remove the liquid from the fibrin by 

 asparating. 



(4) Distribute the fluid in sterile test 

 tubes. 



Sterilization: Not specified. 



Variants: Harvey did not separate the fluid 



from the fibrin and incubated for 48 hours 



to test sterility. 

 References: Besson (1920 p. 34), Harvey 



(1921-22 p. 73). 



1275. Row's Defibrinated Blood Solution 



(Harvey) 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Blood, defibrinated 100.0 cc. 



3. NaCl (1.2% solution) 2200.0 cc. 



Preparation : 



(1) Defibrinated human or rabbit blood. 



(2) Add 10 times its volume distilled 

 water. 



(3) Add 1 volume of the laked blood thus 

 obtained to 2 volumes 1.2% sterile 

 sodium chloride solution. 



Sterilization: Not specified. 

 Use: Cultivation of Leishmania. 

 References: Harvey (1921-22 p. 72), Stitt 

 (1923 p. 52). 



1276. Carpano's Defibrinated Blood 

 Solution 



Constituents : 



1. Distilled water 100.0 cc. 



2. NaCl (c.p.) 7.0 g. 



3. Sodium citrate (c.p.) 7.0 g. 



4. Defibrinated horse blood 

 Preparation: 



(1) Dissolve 2 and 3 in 1. 



(2) Distribute in 1.0 cc. lots into test 

 tubes. 



(3) Remove blood under aseptic condi- 

 tions from the jugular vein of a 

 horse. 



(4) Defibrinate (3) and store in the ice 

 box if not to be used at once. 



(5) Add 9.0 cc. of (4) to each tube of 

 sterile (2) under strictly aseptic 

 conditions. 



(6) Mix well and place in an incubator at 

 25°C. for 24 hours. At the end of 

 this time the mixture is clear. 



Sterilization: Sterilize (2) carefully. 

 (Method not given.) 



Use: Cultivation of Babesia caballi and 

 Nuttallia equi. To inoculate carefully 

 place the end of a sterile fine pointed 

 pipette containing infected blood into 

 the red blood cells in the bottom of the 

 tube, and allow the infected blood to run 

 into the tube. 



Reference: Carpano (1914 p. 43). 



1277. Waksman's Basal Fibrin Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Glycerol 30.0 g. 



3. K2HPO4 10 g. 



4. KCl 0.5 g. 



5. MgS04 0.5 g. 



6. FeS04 0.01 g. 



7. Fibrin 5.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and one of the 

 added nutrients in 1. 



(2) Tube in 10-12 cc. lots. 



(3) Add small pieces of fibrin to each 

 tube. 



Sterilization: Sterilize at 15 pounds for 15 



minutes. 

 Use: To study metabolism of actino- 



mycetes. 

 Added nutrients: The author added 2.0 g. 

 of one of the following: 



NaNOa (NH5)2S04 



NaN02 (NHOiCOa 



Reference: Waksman (1920 p. 3). 



1278. Stoklasa's Glucose Fibrin Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Sodium phosphate 0.5 g. 



3. K2SO4 0.2 g. 



4. MgCl2 0.05 g. 



5. Glucose 2.5 g. 



