374 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



6. CaCOs 7.5 g. 



7. Fibrin 125.0 g. 



Preparation: 



(1) Dissolve 0.5 g. sodium phosphate, 

 0.2 g. K2SO4, and 0.05 g. MgCh in 

 500.0 cc. water. 



(2) Dissolve 2.5 g. glucose in 500.0 cc. 

 water. 



(3) Mix 200.0 cc. (1) and 120.0 cc. of (2). 



(4) Dissolve 7.5 g. CaCOs in (3). 



(5) Distribute in 200.0 cc. lots. 



(6) Add 25.0 g. of fibrin from blood to 

 each flask. 



Sterilization: Method not given. 



Use: To study utilization of nitrogen by 

 Bacillus megatherium, {Bacillus Ellen- 

 bachii) or "Alinit." The author re- 

 ported that more nitrogen was found to 

 be in solution after incubation. 



Variants: The author also gave the follow- 

 ing method of preparation: 



(1) Dissolve 0.5 g. sodium phosphate, 

 0.2 g. potassium sulphate and 0.05 

 g. magnesium chloride in 500.0 cc. 

 water. 



(2) Dissolve 2.5 g. glucose in 500.0 cc. 

 water. 



(3) Mix 400.0 cc. (1) with 240.0 cc. of (2). 



(4) Dissolve 6 in (3). 



(5) Dilute to 4 liters. 



(6) Place 1 liter in a large flask and add 

 40.0 g. fibrin to each flask. 



Reference: Stoklasa (1898 p. 288). 



1279. Beijerinck's Sucrose Fibrin Solution 

 (Percival) 



Constituents: 



1. Water 1000.0 cc. 



2. Cane sugar 50.0 g. 



3. Fibrin 50.0 g. 



4. CaCOs (precipitated) 3.0 g. 



5. Sodium phosphate 0.5 g. 



6. MgS04 0.5 g. 



7. NaCl 0.5 g. 



Preparation : 



(1) Boil 2, 3, 4, 5, 6 and 7 in 1 briskly for 

 2 or 3 minutes. The fibrin is to be 

 finely ground. 

 Sterilization: Not specified. 

 Use: Isolation of Granulobacillus sac- 

 char obutyricus. The medium was in- 

 oculated by the addition of 10.0 to 15.0 

 g. of fresh garden soil while still boiling. 

 Reference: Percival (1920 p. 288). 



1280. Fischer's Blood Meal Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Blood meal 10.0 g. 



3. KH2PO4 1.0 g. 



4. CaCU 0.1 g. 



5. MgS04 0.3 g. 



6. NaCl 0.1 g. 



7. Fe.CU 0.01 g. 



Preparation: 



(1) Dissolve 3, 4, 5, 6 and 7 in a liter of 

 water. 



(2) Distribute in 200.0 cc. lots. 



(3) Add 2.0 g. of blood meal to each flask 

 of (2). 



Sterilization: Sterilize in the autoclave. 



Use: To study decomposition of organic 

 nitrogen by soil forms. Each flask was 

 inoculated with 25.0 cc. of a soil suspen- 

 sion prepared from 100.0 g. soil in 500.0 

 cc. of water. The author reported that 

 the flask containing a trace of acid gave 

 the most ammonia while the one with a 

 trace of alkali showed least ammonia 

 production. 



Variants: The author gave the following 

 variants: 



(a) Dissolve 2.0 g. of blood meal in 200.0 

 cc. of water. Add no salts. 



(b) Dissolve 2.0 g. of blood meal in 200.0 

 cc. of water, no salts, and add 5.0 cc. 

 of a 1 to 10,000 diluted 0.5 normal 

 H0SO4. 



(c) Dissolve 2.0 g. of blood meal in 200.0 

 cc. of water, no salts, and add 5.0 

 cc. of a 1 to 10,000 diluted 0.5 normal 

 NaOH. 



Reference: Fischer (1909 p. 65). 



1281. Remy and Rosing's Blood Albumin 

 Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Blood albumin 50.0 g. 



Preparation : 



(1) Grind dry blood albumin fine. 



(2) Add 1.0 g. sterile (1) to 20.0 cc. of 

 sterile water. 



(3) Distribute into small Erlenmeyer 

 flasks. 



Sterilization: Sterilize (1) twice at 100°C. 

 for 30 minutes. Method of sterilization 

 of the water not given. Sterilize the 



