376 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



of crystalline Na2C03 and 0.1% 

 KNOstoCl). 

 (3) Distribute in 5.0 cc. lots. 

 Sterilization: Sterilize in the autoclave for 



15 to 20 minutes at 0.5 atmosphere. 

 Use: Enrichment medium for cholera 



vibrio. 

 Reference: Ottolenghi (1911 p. 370). 



1285. Jackson's Lactose Bile Solution 



Constituents: 



1. Bile, ox 1000.0 cc. 



2. Lactose (1.0%) 10.0 g. 



Preparation: 



(1) Draw bile, and sterilize undiluted. 

 It may be kept in stock in this 

 manner. 



(2) When ready for use, decant or filter. 



(3) Add 1.0% lactose that has previously 

 been dissolved in small (amount not 

 given) amount of water. 



(4) Mi.x (2) and (3) and tube. 

 Sterilization: Sterilize in autoclave for 30 



minutes at 15 pounds pressure. 

 Use: Enrichment medium used in water 



analysis. 

 Variants: Stitt reported that a 15 to 20.0% 



solution of a good quality of inspissated 



ox bile may be substituted for fresh ox 



bile. 

 References: Jackson (1907 p. 31), Stitt 



(1923 p. 47). 



1286. Meyerstein's Glycerol Bile Solution 



Constituents: 



1. Bile, ox. 



2. Glycerol. 

 Preparation : 



(1) Evaporate ox gall with animal char- 

 coal on a water bath. 



(2) Take up the residue in alcohol. 



(3) Filter. 



(4) Add quite a lot of ether to the some- 

 what concentrated filtrate. This 

 causes the bile acid to precipitate out 

 in silky crystals — so-called crystalline 

 bile. 



(5) Dry and pulverize the crystals. 



(6) Dissolve about 30.0 or 40.0% of (5) in 

 glycerol by heating. 



(7) Place several drops of (6) in test 

 tubes. 



Sterilization: Medium sterilized during the 

 preparation. 



Use: Enrichment of typhoid bacilli from 

 patients blood. Add 1.0 cc. of patients 

 blood to each tube of (6). 



Reference: Meyerstein (1906 p. 1864). 



1287. Thoinot and Masselin's Aqueous 

 Humor Mediimi 

 Constituents: 



1. Aqueous humor. 

 Preparation : 



(1) Obtain the aqueous humor from the 

 eye of an animal by searing the 

 cornea with a hot piece of iron and 

 then puncturing the cornea with a 

 pipette. 



(2) Distribute as desired. 

 Sterilization: Not specified. 

 Use: Cultivation of anthrax bacilli. 

 Reference: Thoinot and Alasselin (1902 



p. 28). 



1288. Ohira and Noguchi's Glucose Ascitic 



Fluid Solution 

 Constituents: 



1. Ringer's Solution 1000.0 cc. 



2. Ascitic fluid 1000.0 cc. 



Preparation : 



(1) Mix equal portions of ascitic fluid and 

 Ringer's solution. (See medium 180). 



(2) Do not adjust the reaction. 

 Sterilization: Method not given. 



Use: Cultivation of Trichomonas of the 

 human mouth. {Tetratrichoinonas hom- 

 inis). 



Reference: Ohira and Noguchi (1917 p. 342). 



1289. Sinton's Glucose Body Fluid Solution 

 Constituents: 



1. Ascitic fluid 100.0 cc. 



2. Glucose (50.0% soln.). 1.5 to 2.0 cc. 

 Preparation: 



(1) Draw off ascitic fluid with strict 

 aseptic precautions into sterile flasks 

 until each contains 100.0 cc. 



(2) Add 1.5 to 2.0 cc. of 50.0% sterile 

 glucose solution to each flask. 



(3) Heat for 30 minutes at 56°C. to kill as 

 much complement as possible. 



Sterilization: Method of sterilization of 

 50.0% glucose solution not given. 



Use: Cultivation of Plasmodium falci- 

 parum. 



Variants: The author used hydrocele fluid 

 instead of ascitic fluid. 



Reference: Sinton (1922-23 p. 205). 



