CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



377 



1290. Tanner's Milk Powder Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Milk Powder, Merrell- 



Soule 100.0 g. 



Preparation: 



(1) Add 2 to 1. 



(2) Beat with an egg beater. 



(3) Filter. 



(4) Distribute in 75.0 cc. quantities. 

 Sterilization: Sterilize in the steamer. 

 Use: General culture medium. Author 



cultivated green fluorescent bacteria from 

 water. 

 Reference: Tanner (1918 p. 83), (1919 p. 

 71). 



1291. Brown and Howe's Milk Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Milk (skim) 500.0 cc. 



3. Sodium citrate 4.0 g. 



Preparation: 



(1) Add 500.0 cc. of milk to a liter of 

 water. 



(2) Add 4.0 g. of sodium citrate and allow 

 to stand for one hour. 



(3) Filter. 



(4) Adjust to pH 6.8. 



(5) Tube. 



Sterilization: Sterilize in Arnold. (A pre- 

 cipitate appears when heated but disap- 

 pears when cool). 



Use: General culture medium. 



Variants: The author used 4.0 g. sodium 

 oxalate instead of 4.0 g. sodium citrate. 

 Citrated milk was found to be more 

 satisfactory than oxalated milk. 



Reference: Brown and Howe (1922 p. 512). 



1292. Boekhout's Sucrose Milk Solution 



Constituents: 



1. Milk 1000.0 cc. 



2. Sucrose (8.0%) 80.0 g. 



Preparation: 



(1) Dissolve 8.0% sucrose in milk. 

 Sterilization: Not specified. 

 Use: Cultivation of dextran formers. 



{Streptococcus hornensis). Organism 



may be isolated from flowers, honey and 



bees. 

 Reference: Boekhout (1900 p. 162). 



1293. Reinsch's Milk Medium 



Constituents: 



1. Milk. 

 Preparation : 



(1) Place 500.0 cc. of fresh cow milk in a 

 separatory funnel and add 1.0 g. 

 NaOH (2.5 cc. solution of 400.0 g. 

 NaOH in a liter). 



(2) Shake well and allow to stand at 

 about 18°C. for 48 hours. 



(3) Remove the nearly transparent milk 

 from the bottom of the funnel and 

 add it to a second separatory funnel. 



(4) Add 250.0 cc. ether and shake well. 



(5) Allow to stand for 48 hours. 



(6) Place the opalescent liquid now in a 

 large sterile flask, plug with cotton 

 and heat to 50°C. 



(7) Place under the receiver of a water 

 suction pump for 3 or 4 hours until all 

 the ether is evaporated. 



Sterilization : Given under preparation. 

 Use: General culture medium. Medium is 



fat free. 

 Variants : 



(a) Migula gave the following method of 

 preparation: 



(1) Obtain milk under aseptic condi- 

 tions in a sterile flask. 



(2) Add 2.0 cc. of chloroform to 100.0 

 cc. of (1). 



(3) Seal the flask with a rubber stopper 

 and shake thoroly. 



(4) Allow to stand for several days. 



(5) Distribute in sterile tubes. 



(6) Incubate for 48 hours. 



(b) Smith prepared the medium as 

 follows: 



(1) Obtain fresh milk and free from fat 

 by running the milk thru a separa- 

 tor or by allowing the cream to rise 

 spontaneously. 



(2) Test the reaction. It should not 

 be more than +2. 



(3) Tube. 



(4) Sterilize at 100°C. by the fractional 

 method. 



A large number of investigators have 

 prepared milk in a manner very 

 similar to that of Smith. Some 

 sterilized the milk in the autoclave. 

 The differences in preparation are not 



