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CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



outstanding enough to warrant an 

 individual discussion of each method, 

 (c) Tanner prepared "Dialyzed Milk" as 

 follows: 



(1) Add 0.1 cc. of hydrogen peroxide to 

 500.0 CO. of milk. 



(2) Allow to stand for 2 hours. 



(3) Add hydrogen peroxide again 

 (amount not specified). 



(4) Allow to stand 4 or 5 hours. 



(5) Dialyze (method not given). 



(6) Dilute the milk one fifth with water. 

 References: Reinsch (1892 p. 31), Migula 



(1901 p. 19), Smith (1902 p. 104), Thoinot 

 and Masselin (1902 p. 27), Committee 

 A.P.H.A. (1905 p. 109), Smith (1905 p. 46), 

 (1905-06 p. 202), Abel (1912 p. 29), Lohnis 

 (1913 p. 20), Tanner (1914 p. 72), Ball 

 (1919 p. 83), Besson (1920 p. 32), Percival 

 (1920 p. 57), Abbott (1921 p. 138), Dopter 

 and Sacquepee (1921 p. 122), Harvey 

 (1921-22 p. 94), Cunningham (1924 p. 16), 

 Park, Williams and Krumwiede (1924 

 p. 120). 



1294. Hiss' Basal Litmus Milk Solution 



Constituents: 



1. Milk 1000.0 cc. 



2. Litmus solution 

 Preparation: 



(1) Tinge milk with litmus solution. 



(2) Dissolve 1.0% of one of the added 

 nutrients in (1). 



Sterilization: Method not given. 



Use: To study fermentation by dysentery 



group. 

 Added nutrients: The author added 1.0% 

 of one of the following: 



glucose dextrin 



maltose mannitol 



sucrose 

 Reference: Hiss (1904-05 p. 31). 



1295. Tanner's Azolitmin Milk Powder 

 Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Milk powder, Merrell- 



Soule 100.0 g. 



3. Azolitmus, Kahlbaum's 

 Preparation: 



(1) Add 100.0 g. of Merrell-Soule's milk 

 powder to 1000.0 cc. distilled water. 



(2) Beat with an egg beater. 



(3) Add sufficient Kahlbaum's azolitmus 

 to give a color. 



(4) Filter. 



(5) Distribute in 75.0 cc. quantities. 

 Sterilization: Method not given. 



Use: General culture medium. Green flu- 

 orescent bacteria of water, B. coli, B. 

 welchii and Bad. acidi lactici were used. 



Variants: Hamilton prepared a similar 

 medium by mixing 1 part purified litmus 

 or azolitmin with 52.7 parts milk powder 

 or 1 part purified azolitmin with 49.6 

 parts milk flour. One part of this mix- 

 ture was dissolved in 9.5 parts water, and 

 the medium was sterilized in the 

 autoclave. 



References: Tanner (1918 p. 83), Hamilton 

 (1921 pp. 43, 44). 



1296. Bacto Litmus Milk (Dehydrated) 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Litmus milk, Bacto 105.0 g. 



Preparation: 



(1) Dissolve 105.0 g. of Bacto litmus milk 



(dehydrated) in 1000.0 cc. distilled 



water. 



Sterilization : Sterilize in the usual manner. 



Use: Cultivation of organisms causing the 



souring of milk and dairy products. 

 Reference: Digestive Ferments Co. (1925 

 p. 13). 



1297. Calandra's Picric Acid Litmus Milk 

 Solution 



Constituents: 



1. Litmus solution. 



2. Picric acid (saturated solution). 

 Preparation : 



(1) Mix 3 parts of a sterile saturated 

 solution of picric acid, 1 part sterile 

 20.0% NaOH solution and 3 parts 

 sterile litmus tincture solution. Do 

 not heat this mixture. 



(2) Add the above sterile indicator mix- 

 ture to sterile milk. 



Sterilization: Sterilize the NaOH, litmus 

 and picric acid separately in the auto- 

 clave. Method of sterilization of milk 

 not given. 



Use: Differentiation between typhoid 

 bacilli and colon bacilli. Author re- 

 ported that typhoid bacilli did not cause a 

 change in color nor cause coagulation. 



