384 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(8) Filter thru a candle. 



(9) Distribute. 



(d) Harvey prepared the medium as 

 follows: 



(1) Add 1.5 cc. strong HCl acid or 

 glacial acetic acid to 1000.0 cc. 

 fresh, slightly warm milk. 



(2) Boil. 



(3) Filter thru well-wetted, thick 

 filter paper. 



(4) Make the reaction of the filtrate 

 neutral to litmus by the addition 

 of dilute sodium carbonate 

 Bolution. 



(5) Filter, while hot thru well-wetted, 

 thick filter paper. 



(6) Add litmus solution to give a deep 

 purple red color. 



(7) Distribute into test tubes or flasks. 



(8) Sterilize. 



References: Grimbert and Legros (1901, p. 

 913), Migula (1901 p. 21), Abel (1912 p. 

 49), Tanner (1919 p. 71), Bezan^on (1920 

 p. 116), Besson (1920 p. 59), Dopter and 

 Sacqu6p6e (1921 p. 123), Harvey (1921-22 

 p. 95). 



1312. Barsiekow's Basal Nutrose Solution 

 (Segin) 



Constituents : 



1. Water 1000.0 cc. 



2. Nutrose 10.0 g. 



3. NaCl 5.0 g. 



4. Litmus tincture 100.0 cc. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Tube. 



(3) Prepare solutions of one of two added 

 nutrients in the litmus tincture. 



(4) Add sufficient quantity of sterile (3) 

 to each sterile tube of (2) to give the 

 desired concentration of fermentable 

 material. 



Sterilization: Sterilize (2) for an hour in 

 the steamer. Sterilize (3) for 15 minutes 

 in the steamer 

 Use : To determine fermentation reactions. 

 Added nutrients and variants : 



(a) Segin added one of the following 

 materials: 



Lactose Erythritol 



Glucose Dulcitol 



Maltose Mannitol 



(b) Hetsch prepared the medium as 

 follows: 



(1) Boil 10.0 g. nutrose, and 5.0 g. 

 NaCl in 1000.0 cc. of distilled water 

 for 2 hours. 



(2) Boil 20.0 g. mannitol or 25.0 g. 

 maltose in 50.0 cc. of Kahlbaum's 

 litmus solution for 10 minutes. 



(3) Prepare solutions (strength not 

 given) of the sugars and alcohols 

 listed. 



(4) Tube in about 10.0 cc. lots and 

 sterilize in flowing steam for J hour. 



(5) Add 1 of (4) to (2). 



(c) Hiss dissolved 10.0 g. nutrose, 4.0 

 cc. of normal NaOH 10.0 cc. of 5.0% 

 litmus solution and one of the follow- 

 ing in 1000.0 cc. distilled water. 



Glucose Dextrin 



Maltose Mannitol 



Sucrose 



(d) Elser and Huntoon prepared the 

 medium as follows: 



(1) Dissolve 10.0 g. nutrose and 5.0 g. 

 NaCl in 1000.0 cc. distilled water. 



(2) Add 5.0 to 7.5 cc. of a watery solu- 

 tion of Merk's highly sensitized 

 litmus. 



(3) Sterilize, in usual manner (exact 

 method not specified). 



(4) Prepare a 10.0% solution of one of 

 the following in distilled water. 



Glucose Dulcitol 



Galactose Inulin 



Levulose Dextrin 



Lactose Maltose 



Sucrose Mannitol 



(5) Sterilize (4) at 100°C. for 10 

 minutes. 



(6) Mix (5) and (3). 



(7) Tube in sterile tubes. 



(8) Incubate for 3 days to detect acci- 

 dental contamination. 



(e) Tanner prepared the medium as 

 follows: 



(1) Dissolve 10.0 g. nutrose and 5.0 g. 

 NaCl in 750 cc. distilled water. 



(2) Dissolve 10.0 g. of any desired 

 carbohydrates, alcohol, etc. in 

 250.0 cc. of water. 



(3) Add litmus solution to (2) to give 

 an amethyst color. 



(4) Cool (1) and (3). 



(5) Mix (1) and (3). 



