CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



385 



(6) Tube. 



(7) Sterilization not specified. 



(f) Klimmer gave the following method 

 of preparation: 



(1) Mix 10.0 g. of nutrose and 4.0 g. 

 NaCl in a mortar and add water, 

 drop by drop until a thick paste is 

 obtained. 



(2) Steam for 1 to 2 hours. 



(3) Allow to settle. 



(4) Carefully decant and filter thru a 

 folded filter paper. 



(5) Dissolve one of the following in 50.0 

 cc. of Kubel and Tiemann's litmus 

 solution. 



lactose 10.0 g. 



glucose 10.0 g. 



maltose 20.0 g. 



sucrose 20.0 g. 



(6) Add (5) to (4). 



(7) Add 0.1 normal NaOH until the 

 medium is neutral to the litmus. 



(8) Distribute in 6.0 cc. quantities in 

 tubes. 



(9) Sterilize for 25 to 30 minutes in the 

 steamer. 



References: Segin (1903, p. 203), Hetsch 

 (1903, p. 580), Hiss (1904-05, p. 31), 

 Elser and Huntoon (1909, p. 404), Tanner 

 (1919, p. 59), Besson (1920, p. 59), Harvey 

 (1921-22, p. 97), Klimmer (1923, p. 211). 



1313. Purwin and McNutt's Basal Nutrose 

 Solution 



Constituents : 



1. Water 990.0 cc. 



2. Nutrose 10.0 g. 



3. NaCl 0.5 g. 



4. Andrade indicator (1.0%) 

 Preparation : 



(1) Mix 2 and 3 with 1 and allow to stand 

 over night. 



(2) Adjust the reaction to pH 7.7. 



(3) Steam in the Arnold for 2 hours. 



(4) Filter thru cotton. 



(5) Add 1.0% Andrade indicator. 



(6) Adjust to a very slight pink if 

 necessary. 



(7) Add one of the added nutrients. 

 Sterilization: Sterilize in the Arnold on 



each of 3 successive days. 

 Use: Sugar free broth to study fer- 

 mentation. 



Added nutrients: The authors added any 



desired carbohydrates. 

 Reference: Purwin and McNutt (1924 p. 



297). 



1314. Th5ni and Allemann's Nutrose 

 Solution 



Constituents: 



1. Water.., 3000.0 cc. 



2. Nutrose...,, 30.0 g. 



Preparation : 



(1) Dissolve 30.0 g. of sodium caseinate 

 (nutrose) in 3 liters of water. 

 Sterilization: Not specified. 

 Use: Cultivation of Bacillus putrificus. 



Inoculate and incubate under anaerobic 



conditions. 

 Reference : Thoni and AUemann (1916 



p. 101). 



1315. Ficker and Hoffmann's Caffeine 

 Nutrose Solution 



Constituents: 



1. Distilled water. . , 200.0 cc. 



2. Nutrose 10.0 g. 



3. Caffeine 5.0 g. 



4. Crystal violet (Hochst) 0.1 g. 



Preparation : 



(1) Dissolve 10.0 g. nutrose in 200.0 cc. 

 distilled water by boiling on the 

 water bath for several hours. 



(2) Make up the loss of water after 

 cooling. 



(3) Dissolve 5.0 g. caffeine in 20.0 cc. 

 distilled water at about 80°C. Cool 

 to 55 to 60°C. 



(4) Dissolve exactly 0.1 g. Hochst's 

 crystal violet in 100.0 cc. distilled 

 water. 



Sterilization: Not specified. 



Use: Enrichment of typhoid and cholera 

 bacilli from water and other materials. 

 The author added 900.0 cc. of the water 

 under investigation in a flask. Pour 

 sterile (2) into the flask containing (3). 

 (Do not reverse the order). Mix thoroly. 

 Add this mixture to the water under 

 investigation and add 10.0 cc. of (4). 

 Shake thoroly. Incubate for 12 to 13 

 hours at 37°C. and then plate on medium. 

 Reitz used the medium for the bacteri- 

 ological examination of butter. He 

 added 200.0 g. of butter under investiga- 



