386 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



tion, melted at 20 or 30°C., to the medium 

 instead of 900.0 cc. of water as above. 

 References: Ficker and Hoffmann (1904 p. 

 268), Reitz (1906 p. 722). 



1316. Loeffler's Lactose Nutrose Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Nutrose., 10.0 g. 



3. Lactose 20.0 g. 



4. Malachite green (2.0% soln). 50.0 cc. 

 Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Add 5.0% of a 2.0% malachite green 

 solution to (1). 



Sterilization: Not specified. 



Use: Enrichment of typhoid bacilli from 



faeces. 

 Variants: Klimmer prepared the medium 



as follows: 



(1) Dissolve 10.0 g. nutrose in 1 liter hot 

 distilled water. 



(2) Add 20.0 g. lactose to (1). 



(3) Flask in 100.0 cc. quantities. 



(4) Sterilize on each of 3 successive days 

 for 10 minutes in streaming steam. 



(5) Add 5.0 cc. of a 2.0% solution of 120 

 Hochet malachite green to each 100.0 

 cc. lot. 



References: Loeffler (1906, p. 289-295), 

 Klimmer (1923, p. 213). 



1317. Hill's Artificial Milk Medium 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Nutrose.... 20.4 g. 



3. Lactose 10.0 g. 



Preparation : 



(1) Mix 1, 2 and 3 and allow to stand for 

 12 hours in the cold. 



(2) Shake thoroly and filter thru cotton. 



(3) Tube. 



Sterilization: Tube and sterilize at 110° for 



20 minutes. 

 Use: Substitute for milk. General culture 



medium. 

 Reference: Hill (1909 p. 379). 



1318. Doerr's Mannitol Nutrose Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Mannitol 10.0 g. 



3. NaCl 5.0 g. 



4. Nutrose 10.0 g. 



5. Litmus solution, Kahlbaum 30.0 cc. 

 Preparation : 



(1) Add 2, 3 and 4 to 1. 



(2) Heat in the water bath until solution 

 is complete. 



(3) Filter. 



(4) Add 30.0 cc. of Kahlbaum's Litmus 

 Solution. 



(5) Distribute into flasks or fermentation 

 tubes. 



Sterilization: Sterilize in streaming steam 

 for 15 minutes on two successive days. 



Use: Differentiation of colon typhoid and 

 dysentery group. The author reported 

 that Flexner's strains gave red coloration 

 after 24 hours. Shiga group caused no 

 change. Typhoid and coli forms gave 

 the same reaction as in dextrose-nutrose 

 medium, coli causing a precipitation of 

 the nutrose in 24 hours while the typhoid 

 organisms required a longer period to 

 produce precipitation. 



Variants : Lehman prepared the medium as 

 follows: 



(1) Dissolve 4.0 g. Nutrose (Kahlbaum) 

 and 2.0 g. NaCl in 400.0 cc. distilled 

 water. 



(2) Sterilize for 45 minutes in the 

 steamer. 



(3) Filter off 300.0 cc. immediately after 

 sterilization. 



(4) Dissolve 3.0 g. mannitol in 15.0 cc. of 

 litmus solution by heating slightly. 



(5) Mix (4) and (3). 



(6) Distribute into 4.0 cc. lots and 

 sterilize for 20 minutes in the steamer. 



Reference: Doerr (1903 p. 395), Lehman 

 (1916-17 p. 102). 



1319. Whittaker's Lactose Caseinogen 

 Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Caseinogen 15.0 g. 



3. Lactose 10.0 g. 



4. CaClo 0.1 g. 



Preparation : 



(1) Prepare a 1.0% solution of NaOH in 

 distilled water. 



(2) Dissolve 15.0 g. of caseinogen in 100.0 

 cc. of (1). (18 to 24 hours may be 

 required for this.) 



