CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



387 



(3) Dilute to 900.0 cc. with distilled 

 water. 



(4) Add 10.0 g. lactose and 0.1 g. CaCh 

 to (3). 



(5) Make the solution up to 1000.0 cc. 

 by the addition of distilled water. 



(6) Neutralize and make + 0.3 with 

 normal HCl, using phenolphthalein 

 as an indicator. 



Sterilization: Sterilize in the autoclave at 



107° for 20 minutes. 

 Use: Substitute for milk. General culture 



medium. 

 Reference: Whittaker (1912 p. 162). 



1320. Waksman's Basal Casein Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Glycerol 30.0 g. 



3. K2HPO4 1.0 g. 



4. KCl 0.5 g. 



5. MgS04 0.5 g. 



6. FeS04 0.01 g. 



7. Casein 5.0 g. 



Preparation: 



(1) Dissolve 2, 3, 4, 5, 6 and one of the 

 added nutrients in 1. 



(2) Dissolve casein in N/10 NaOH and 

 add to (1). 



(3) Tube in 10 to 12 cc. lots. 

 Sterilization: Sterilize at 15 pounds for 15 



minutes. 

 Use: To study metabolism of actinomy- 



cetes. 

 Added nutrients: The author added 2.0 g. 

 of one of the following: 



NaXOa (NH4)2S04 



NaXOj (NH 4)200, 



Reference: Waksman (1920 p. 3). 



1321. Seliber's Basal Casein Solution 

 (Harvey) 



Same as medium 743 but not containing 

 peptone. 



1322. Laxa's Lactic Acid Casein Solution 



Constituents: 



1. Water 1000.0 cc. 



2. NaCl 5.0 g. 



3. CaClj 0.1 g. 



4. MgCIa 0.2 g. 



5. Potassium phosphate 



(neutral) 2.5 g. 



6. Lactic acid 3.0 g. 



7. Casein 30.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Suspend 30.0 g. of casein in (1). 

 Sterilization: Method not given. 

 Use : Cultivation of oidium. 

 Reference: Laxa (1901-02 p. 129). 



1323. Baginsky's Casein Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Casein 1000.0 g. 



Preparation : 



(1) Add 20.0 g. of casein to 20.0 cc. of 

 water (or nutrient solution, composi- 

 tion not given). 



(2) Distribute into sterile flasks. 

 Sterilization: Sterilize in the steamer. 

 Use: To study fermentation by B. lactis. 

 Reference: Baginsky (1888 pp. 43'^462). 



1324. Harvey's Alkaline Casein Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Casein 20.0 g. 



3. NaOH (normal) 10.0 cc. 



Preparation : 



(1) Mix 1, 2 and 3. 

 Sterilization: Not specified. 

 Use: Cultivation of anaerobic organisms. 



Author specified that nutrose or any other 



casein product could be used instead of 



casein. 

 Reference: Harvey (1921-22 p. 96). 



1325. Geilinger's Basal Urine Solution 



Constituents: 



1. Urine, cow. 

 Preparation: 



(1) Mix one of the added nutrients with 

 sterile cow's urine. 

 Sterilization: Sterilize cow's urine by heat- 

 ing for one hour at 0.5 atmosphere pres- 

 sure or preferably by filtering thru a 

 Chamberland clay candle filter. 

 Use: Cultivation of organisms capable of 



splitting urea, Bac. urea. 

 Added nutrients and variants : 



(a) The author added 1.0 cc. of one of the 

 following to each 20.0 cc. of sterile 

 urine. 



The added nutrients were prepared as 

 follows: 



(a) Straw infusion. 



(1) Mix 9 parts by weight of 

 water with 1 part by weight 



