388 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



of finely chopped straw and 

 heat in steamer for one 

 hour. 



(2) Filter. 



(3) Sterilize for 45 minutes at 

 0.5 atmosphere pressure. 



(b) Feces infusion. 



(1) Mix one part cow feces 

 with 4 parts water. 



(2) Heat for one hour in the 

 steamer. 



(3) Filter. 



(4) Sterilize for 45 minutes 

 under 0.5 atmosphere pres- 

 sure. 



(c) Peptone solution. 



(1) Prepare and filter a 10.0% 

 peptone solution. 



(2) Sterilize (method not 

 given). 



(b) The author used cow's urine without 

 any additions. 

 Reference: Geilinger (1917 p. 246). 



1326, Bokorny's Sucrose Urine Solution 



Constituents : 



1. Urine 1000.0 cc. 



2. Sucrose SO.O g. 



Preparation : 



(1) Add 80.0 g. sucrose to 1000.0 cc. of 

 undiluted and not neutralized urine. 



Sterilization: Not specified. 



Use: To study the growth of yeast. The 

 author reported that neutralized urine 

 showed better growth than non-neutral- 

 ized. The effect of diluting the urine was 

 that of diluting the food materials. 

 Growth best if diluted urine be neutral- 

 ized. 



Variants : The author added 80.0 g. of sucrose 

 to 1000.0 cc. of urine prepared in the 

 following manner: 



(a) Undiluted urine neutralized with 

 K5HPO4. 



(b) Undiluted urine partially neutral- 

 ized. 



(c) Unneutralized urine mixed with an 

 equal volume of water. 



(d) Neutralize urine with K2HPO4 and 

 then add an equal amount of water. 



(e) Neutralize urine with K2HPO4 and 

 dilute with 3 volumes of water. 



(f) Dilute unneutralized urine with 5 

 times water. 



(g) Neutralize urine with K2HPO4 and 

 dilute 5 times with water. 

 Reference: Bokorny (1920 p. 27). 



1327, Besson's Urine Medium (Tanner) 



Constituents : 



1. Urine. 

 Preparation : 



(1) Boil some recently passed urine. 



(2) If the reaction is markedly alkaline 

 after boiling, add a little tartaric acid 

 solution. Use litmus paper as an 

 indicator. 



(3) Filter. 



(4) Tube. 



Sterilization: Sterilize at 155°C. It is 

 better to filter thru a Chamberland 

 bougie. 



Use: General culture medium. 



Variants : The following variants have been 

 given by the following authors: 



(a) Besson — 



(1) Collect urine under aseptic condi- 

 tions in sterile flasks. 



(2) Distribute in tubes. 



(3) Incubate for 24 hours at 37°C. to 

 test sterility. 



(b) Besson filtered fresh urine thru a 

 Chamberland filter. 



(c) Besson — 



(1) Boil fresh urine. 



(2) Neutralize to litmus by the addition 

 of tartaric acid if necessary. 



(3) Filter. 



(4) Tube. 



(5) Sterilize at 115°C. 



(d) Harvey — 



(1) Collect urine fresh. 



(2) Boil. 



(3) Filter. 



(4) Sterilize in the steamer or auto- 

 clave. 



(e) Harvey — 



(1) Distribute into test tubes freshly 

 passed urine diluted to specific 

 gravity 1.010. 



(2) Sterilize in the steamer or auto- 

 clave. 



References: Tanner (1919 p. 58), Besson 

 (1920 p. 33), Harvey (1921-22 p. 84). 



1328. Burri and Stutzer's Nitrate Feces 

 Solution 



Constituents: 

 1. Water 



1000.0 cc. 



