CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



389 



2. Feces (horse) 50.0 g. 



3. NaNOa 3.2 g. 



Preparation: 



(1) Mix 1, 2 and 3. 

 Sterilization: Do not sterilize. 

 Use: Enrichment of nitrate reducers, and 



to stud)' denitrification. 

 Variants : 



(a) Ampola and Garino specified cow 

 feces instead of horse. 



(b) Jensen mixed 10.0 g. NaNO,, 100.0 g. 

 cow or horse feces and 0.0 or 20.0 cc. 

 of glycerol. He reported that the 

 presence of glycerol aided denitri- 

 fication. 



References: Burri and Stutzer (1895 p. 261), 

 Ampola and Garino (1896 p. 671), Jensen 

 (1897 p. 693). 



1329. Dimitroff's Fecal Infusion 



Constituents: 



1. Tap water 1000.0 cc. 



2. Feces (human) (1.0 to 



2.0%) 10.0 to 20.0 g. 



Preparation : 



(1) Prepare an emulsion of 1.0 to 2.0% 

 human feces in tap water. 

 Sterilization: Method not given. 

 Use : Cultivation of Leptospira biflexa. 

 Reference: Dimitroff (1927 p. 511). 



1330. Dunham's Meat Infusion Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Beef 500.0 g. 



Preparation : 



(1) Boil finely chopped beef with a 

 double weight of water for 2 hours. 



(2) Filter. 



(3) Make slightly alkaline; 0.5% NaCl 

 may be added if desired. 



Sterilization: Not specified. 



Use: Dunham used the solution for the 

 detection of cholera bacilli. He reported 

 that the cholera vibrio gave a red ring 

 where the H2SO4 and medium met when 

 concentrated H2SO4 was poured down the 

 side of the tube containing a culture. 

 Similar media were used by different 

 investigators for a variety of purposes. 



Variants: The following investigators pre- 

 pared media as follows: 

 (a) Migula — 

 (1) Mix 500.0 g. of finely chopped lean 



beef with one liter of water, and 

 allow to stand in the ice box for 12 

 to 24 hours. 



(2) Press the liquid thru a towel and 

 make up the volume to one liter. 



(3) Boil in the steam cooker for 30 

 minutes. 



(4) The infusion may be boiled for an 

 hour before removing the meat, 

 and then filtered thru paper. If 

 the liquid is still red, boil again for 

 15 minutes. 



(5) Distribute in flasks. 



(6) Boil for an hour to sterilize. 



(7) Store until ready for use. Seal the 

 flask with paraffin, gum arable or a 

 beaker if it is to be stored for a 

 long time. 



(b) Abel- 



(1) Chop 500.0 g. of fat-free meat and 

 add to a liter of water at 50°C. 



(2) Keep at 50°C. for 30 minutes and 

 then boil for 30 to 45 minutes. 



(3) Filter or strain the fluid from the 

 meat. 



(4) Make up the fluid to one liter. 



(5) Sterilize on each of 3 successive 

 days in the steamer, or autoclave, 

 for 15 minutes at 120°C. 



(c) Linde — 



(1) Chop 500.0 g. of beef into small 

 pieces. 



(2) Boil with one liter of water. 



(3) Filter. 



(4) Use filtrate without any additions 

 or add a concentrated Na2C03 

 solution to give alkaline reaction. 



(5) Sterilization not specified. 



(d) Davis and Ferry used beef infusion 

 (or a 1.0% meat extract solution) as a 

 basic solution and added one of the 

 following to 1000.0 cc. of the basic 

 solution: 



Cystine 0.5 g 



Tryptophane 0.6 g 



Tyrosine., 1-25 g 



Glutaminic acid hydrochloride. 2.5 g 



Histidine dichloride 0.5 g 



Leucine 3.0 g 



Glycocoll 0.75 g 



Sodium asparaginate 1.5 g 



Glucoseamine hydrochloride... . 2.0 g 



fCreatine 0.2 g 



[Creatinine 0.15 g 



