390 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



fXanthin 0.05 g. 



\Hypoxanthia 0.05 g. 



NaCl 4.0 g. 



K2HPO4 3.0 g. 



lMgS04 0.4 g. 



[KNO3 0.2 g. 



These media were used for the culti- 

 vation of Bact. diphtheriae and toxin 

 production by it. 

 (e) Waksman studied the metabolism of 

 actinomyces in media prepared as 

 follows: 



(1) Boil beef in water, filter and 

 sterilize. 



(2) Inoculate with B. coli communor, 

 incubate at 37° for 24 hours. 



(3) Boil for 10 minutes, adjust to pH 

 = 7.6 to 7.7. Boil again, filter and 

 sterilize. 



(4) Add 1.0% of any desired carbo- 

 hydrate, alcohol, etc., and 1.0% 

 of Andrade indicator. 



(5) Distribute in fermentation tubes. 



(6) Steam in Arnold for 30 minutes to 

 sterilize. 



The author reported that the actin- 

 omycetes developed in the infusion 

 alone without the addition of other 

 nutrients. 



(f) Besson — 



(1) Pour 1000.0 cc. of water on 500.0 g. 

 of finely chopped beef, and place 

 in the ice box for 12 hours. 



(2) Stir the mixture well. 



(3) Filter thru a cloth and press the 

 meat free from juice. 



(4) Filter the juice thru paper. 



(5) Add 5.0 g. NaCl. 



(6) Boil. 



(7) Make slightly alkaline or neutral- 

 ize to litmus by the addition of 

 soda. 



(8) Heat at 115 to 117°C. for 5 minutes. 



(9) Filter until clear. 



(10) Make up to 1000.0 cc. by the addi- 

 tion of distilled water. 



(11) Distribute as desired. 



(12) Sterilize at 110 to 115°C. for 20 

 minutes. 



(g) Giltner— 



(1) Add 500.0 g. of tap water to 500.0 

 g. finely chopped fresh lean beef in 

 3.5 liter agate ware pail. 



(2) Mix thoroly and allow to stand in 



a cool place (refrigerator) for not 

 more than 16 to 24 hours. 



(3) Strain the infusion thru a cheese 

 cloth, thoroly pressing out all the 

 juice. 



(4) Make up to 500.0 cc. by the addi- 

 tion of tap water if necessary. 



(5) Place (4) in a sterile liter Erlen- 

 meyer flask. 



(6) Heat in the autoclave at 120°C. 

 for 30 minutes. 



(h) Harvey — 



(1) Mince finely fat-free beef. 



Note: Veal, chicken, ox or 

 horse heart, horse flesh, rabbit 

 flesh, fish, blood, placenta, liver, 

 spleen, kidneys, brain and vege- 

 table materials, such as yeast, 

 wheat, etc., may serve to furnish 

 the extract used as basis for the 

 medium. 



(2) Add 500.0 g. to 1000.0 cc. distilled 

 water or clear tap water. 



(3) Heat the mixture 20 minutes over 

 a free flame, at a temperature not 

 exceeding 50°C. 



Note: Or simply keep in a cool 

 place over night. 



(4) Skim off fat floating on the surface. 



(5) Raise the temperature to boiling 

 point. 



(6) Boil 10 minutes. 



(7) Pour the mixture on to a wet, 

 thick, clean cloth. 



(8) Add sodium chloride 5.0 g. to the 

 filtrate. 



(9) Steam 45 minutes. 



(10) Bring the volume up to 1000.0 cc. 

 by the addition of water. 



(11) Estimate and adjust the reaction. 



(12) Steam 30 minutes. 



(13) Filter, while hot, thru well-wetted, 

 thick filter paper. 



References: Dunham (1887 p. 338), Migula 

 (1901 p. 13), Abel (1912 p. 13), Linde 

 (1913 p. 386), Davis and Ferry (1919 p. 

 235), Waksman (1919 p. 316), Besson 

 (1920 p. 28), Dopter and Sacquepee 

 (1921 p. 120), Giltner (1921 p. 27), Harvey 

 (1921-22 p. 94). 



1331. Stutzer and Hartleb's Nitrite Infusion 

 Solution 



Constituents ; 

 1. Water... 



2000.0 cc 



