392 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(6) Decant carefully and strain thru a 

 straining cloth. 



(7) Filter thru paper. 

 Sterilization: Not specified. 



Use : Use as meat water in the preparation 



of media. 

 Reference: Klimmer (1923 p. 172). 



1336. Szasz's Blood Clot Infusion 

 Constituents: 



1. Distilled water 2000.0 cc. 



2. Blood clot , 1000.0 g. 



Preparation : 



(1) Take 1000.0 g. of blood from which the 

 serum has been separated, coagulated 

 blood or blood clots and add 2000.0 

 cc. of distilled water. 



(2) Boil for a short time. (Time not 

 specified.) 



(3) Separate the clot into pieces the size 

 of a nut and boil longer. Do not cut 

 the clot before heating. Take care 

 that the blood clot does not sink to 

 the bottom of the kettle and burn. 

 Place the kettle or container in which 

 the boiling is taking place over a free 

 flame. It may be stirred with a 

 wooden spoon. A linen towel may 

 be placed in the bottom of the con- 

 tainer and extend part way up the 

 side walls or all the way up and be 

 fastened with a cord. This is the 

 simplest way to prevent burning. 



(4) Filter thru a large linen towel. 



(5) The bouillon may be clarified with 

 egg white. 



Sterilization: Not specified. 



Use: Inexpensive medium. Meat infusion 



or extract substitute. 

 Variants : 



(a) The author prepared a similar me- 

 dium as follows: 



(1) Divide the blood clots into pieces 

 the size of a walnut or hazel nut by 

 means of a piece of wood or hands. 



(2) To the clot obtained from every 

 kilogram of blood, add 1.5 liters 

 of water. 



(3) Mix well and allow to stand in the 

 cold for 20 to 24 hours, stirring 

 several times. 



(4) Filter thru a coarse linen cloth and 

 boil the filtrate until brown clumps 

 are formed and the liquid is yellow. 



(5) Make up to the original volume, 

 adjust the reaction as desired in 

 the same manner as for meat 

 extract. 



(6) The bouillon may be filtered until 

 clear. 



(7) Usually 0.5% glycerol is added. 



(8) Sterilization not specified. 



(b) Stefanopoulo cultivated Spirochaeta 

 icterohemorragiae on a medium pre- 

 pared as follows: 



(1) Separate the serum from the clot 

 of coagulated horse blood. 



(2) Press the clot thru a fine wire gauze. 



(3) Dilute with two times its volume 

 with physiological salt solution 

 prepared by dissolving 8.0 g. of 

 NaCl in 1000.0 cc. of water. 



(4) Heat for 15 minutes at 80°C. 



(5) Filter thru paper, and then on a 

 Chamberland filter. 



(6) Distribute in sterile tubes and cover 

 with a layer of sterile vaseline. 



References: Szasz (1914-15 p. 491), (1915- 

 16 p. Ill), Stefanopoulo (1921 p. 813). 



1337. Wellman's Placenta Infusion 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Placenta, human 1000.0 g. 



Preparation : 



(1) Grind fresh human placenta thoroly 

 in a meat chopping machine after 

 first washing out the blood by running 

 sterile salt solution thru the attached 

 vessel. 



(2) To each kilogram of macerated 

 placental tissue add 1 liter of distilled 

 water. 



(3) Infuse for 48 hours in the ice box. 



(4) Tube sterile (3). 



(5) Store the medium at 40-41 °C. for 2 

 days before use to inactivate the 

 complement. 



Sterilization: Pass thru a No. N Berkefeld 

 filter that will hold back ordinary bac- 

 teria. To facilitate this fill the cylinder 

 of the filter with a clean fine sterile sand 

 until the cylinder is completely covered. 



Use: Cultivation of parasitic bacteria. 

 Author used Bacillus leprae and tubercle 

 bacilli. 



Variants : 



(a) Groer and Srnka studied the produc- 



