394 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Preparation : 



(1) Obtain beef salivary glands from the 

 slaughter house. 



(2) Cut in small pieces with a meat 

 chopper. 



(3) Add 5 volumes of water. 



(4) Filter thru blotting paper. 



(5) Distribute sterile (4) into sterile 

 tubes. 



Sterilization: Filter thru a Chamberland 



filter to sterilize. 

 Use : Medium when inoculated with plague 



containing material contained round cells 



after two days. 

 Variants : 



(a) The author added 3.0% NaCl or 2.0 

 to 3.0% NaCl plus 0.2 to 0.5 g. NaOH 

 or KOH per liter. 



(b) Mayer prepared a similar medium as 

 follows : 



(1) Chop fresh salivary glands in a 

 meat chopping machine. 



(2) Mix with an equal weight of water, 



(3) Infuse on ice for 24 hours after 

 stirring strongly. 



(4) Press the mass in a meat press. 



(5) Sterilize the thick, slimy liquid in 

 streaming steam for 30 minutes. 

 Mayer reported that a meat extract 

 medium generally gave better 

 growth of parasitic and saprophytic 

 bacteria. 



References: Nencki, Sieber and Wyzni- 

 kiewicz (1898 p. 530), Mayer (1899 p. 

 747). 



1342. Graham-Smith's Heart Infusion 



Constituents : 



1. Distilled water 250.0 cc. 



2. Heart, bullock 100.0 g. 



Preparation : 



(1) Remove the fat and vessels from 

 fresh bullock's heart muscle. 



(2) Pass thru a mincing machine. 



(3) To each 100.0 g. of (2) add 250.0 cc. 

 of water. 



(4) Boil gently for 99 minutes. 



(5) Filter. 



Sterilization: Sterilize for 20 minutes on 3 

 successive days in a steam sterilizer. 



Use: General culture medium. Robertson 

 and Davis used a similar medium to study 

 the influence of vitamines on bacterial 

 growth. They reported that the medium 

 did not give continued growth of yeast. 



Variants: Robertson and Davis prepared a 

 similar medium in the following manner: 



(1) Grind lean beef heart finely and just 

 cover with sterile distilled water. 



(2) Place in ice box 18 hours. 



(3) Pass thru Berkefeld filter. 



(4) Mix (3) with sterile (method of 

 sterilization not given) physiological 

 salt solution. 



Reference: Graham-Smith (1920-21 p. 136), 

 Robertson and Davis (1923 p. 154). 



1343, Robertson and Davis' Heart Infusion 



Medium 



Constituents: 



1. Water, sterile distilled 1000.0 cc. 



2. Asparagin (Merck) 3.4 g. 



3. CaCU 0.1 g. 



4. Glucose 20.0 g. 



5. MgS04 0.2 g. 



6. K2HPO4 1.0 g, 



7. NaCl 5.0 g. 



8. Heart, beef. 

 Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1 by 

 boiling 3 minutes. 



(2) Restore original volume with sterile 

 distilled water. 



(3) Adjust reaction to pH = 7.4 and tube. 



(4) Grind lean beef heart finely and just 

 cover with sterile distilled water. 



(5) Place in ice box 18 hours. 



(6) Pass thru Berkefeled filter. 



(7) Add various amounts of (6) to each 

 tube of sterile (3). 



Sterilization: Autoclave (3) at 20 pounds 

 pressure for 30 minutes. Heart infusion 

 is sterilized by filtration thru Berkefeld 

 filter. 



Use: To study influence of vitamins on 

 bacterial growth. Author reported a 

 luxuriant growth of yeast in this medium. 



Reference: Robertson and Davis (1923 p. 

 154). 



1344, Mereshkowsky's Egg White Infusion 



Constituents: 



1. Water 1000.0 cc. 



2. Egg albumin 100.0 g. 



Preparation : 



(1) Remove the shell from hard boiled 

 hen eggs. 



(2) Cut the egg in 4 lengthwise strips 

 and remove the egg yolk. 



