CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



395 



(3) Pass the egg white thru a meat grind- 

 ing machine. 



(4) To each 100.0 g. of finely chopped egg 

 white add 1000.0 cc. of water. 



(5) Place in a flask and plug. 



(6) Autoclave for 15 minutes under one 

 atmosphere of pressure. 



(7) Filter thru cotton, and then thru 

 paper. 



(8) Distribute into test tubes. 

 Sterilization: Method not given. 



Use: Cultivation of Bacillus Danysz. The 

 author reported that Bacillus Danysz did 

 not lose its virulence on repeated cultiva- 

 tion on this medium. 



Reference: Mershkowsky (1912 p. 394). 



1345. Proca's Spleen Infusion Solution 



Constituents: 



1. Ringer solution 1000.0 cc. 



2. Spleen 400.0 g. 



Preparation: 



(1) Add 400.0 g. of finely chopped fresh 

 beef spleen to 1000.0 cc. of Ringer's 

 solution (see medium ^180). 



(2) Heat at 115°C. for 30 minutes. 



(3) Filter while hot thru paper. 



(4) Distribute in tubes or flasks. (The 

 filtrate may be solidfied with 1.4% 

 agar or 10.0% gelatin). 



Sterilization: Sterilize in the autoclave. 

 Use: General culture medium. 

 Reference: Proca (1924 p. 1164). 



1346. Harde and Hauser's Fish Infusion 



Constituents: 



1. Water 1000.0 cc. 



2. Fish (Whiting) 500.0 g. 



Preparation: 



(1) Boil 500.0 g. of chopped whiting fish 

 with 1 liter of water for 20 minutes. 



(2) Filter thru paper. 



(3) Reaction is neutral to litmus. 

 Sterilization: Sterilize at 120^ for 20 



minutes. 

 Use: Substitute for beef media and as a 



general culture medium. 

 Reference: Harde and Hauser (1919 p. 



1259). 



1347. Pergola's Mussel Infusion 



Constituents : 



1. Water 1000.0 cc. 



2. Mussels 500.0 g. 



Preparation : 



(1) Remove mussels from their shells. 



(2) Work (1) into a pulp and mi.x with a 

 double weight of water. 



(3) Place in the autoclave for 15 to 20 

 minutes under 1 atmosphere pressure. 



(4) Filter thru filter paper until a not 

 exccessive turbid, at the most a 

 slightly opalescent, liquid is obtained. 



(5) Place in the autoclave for 30 minutes 

 again under 1 atmosphere pressure. 



(6) Filter. The filtrate is a clear amber 

 yellow. 



(7) Distribute into sterile tubes. 

 Sterilization: The medium may be steri- 

 lized in the autoclave if necessary. 



Use: Cultivation of typhoid, cholera and 

 other intestinal forms. Author reported 

 that the cholera vibrio lost its character- 

 istic appearance on this medium. 



Variants : 



(a) The author used oysters instead of 

 mussels. 



(b) The medium was prepared as follows: 



(1) Remove oysters or mussels from 

 their shell. 



(2) Work (1) into a pulp and mix with a 

 double weight of sterile phyiologi- 

 cal salt solution. 



(3) Allow the mixture to stand for 24 to 

 48 hours at 15°C. 



(4) Pour off the liquid, filter thru cotton 

 then thru paper and finally thru a 

 Berkefeld candle. 



(5) This gives a yellowish amber fluid. 

 The author reported that the organ- 

 isms failed to develop on this 

 medium. 



Reference: Pergola (1912 p. 171). 



1343. Besson's Chicken Infusion (Tanner) 



Constituents: 



1. Water 1000.0 cc. 



2. Chicken 500.0 g. 



Preparation: 



(1) Soak 500.0 g. of lean chicken meat in 

 1000.0 cc. water. 



(2) Filter. 

 Sterilization: Not specified. 

 Use: Substitute for beef infusion. 

 Reference: Tanner (1917 p. 46). 



