396 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



1349. Biisgen and Hoflich's Meat Extract 



Solution (Linde) 

 Constituents: 



1. Water 1000.0 cc. 



2. Meat extract (0.5%) ........ 5.0 g. 



Preparation : 



(1) Dissolve 2 in 1. 

 Sterilization: Not specified. 

 Use : Enrichment of Cladothrix, Cladothrix 



dichotoma. Cultivation of other 



organisms. 

 Variants : 



(a) The author added a concentrated 

 Na2C03 solution to obtain an alkaline 

 reaction. 



(b) Zikes cultivated cladothrix and 

 Sphaerotilus natans in a medium 

 containing 0.5, 0.25, 0.125, 0.063 or 

 0.031% meat extract. 



(c) Besson prepared the medium as 

 follows: 



(1) Dissolve 0.5% Liebig's meat ex- 

 tract (or 20.0 g. Cibils meat extract) 

 in 1000.0 cc. of water. 



(2) Make alkaline if necessary. 



(3) Autoclave at 115 to 117° for 5 

 minutes. 



(4) Filter while hot thru a wet filter. 



(5) Tube. 



(6) Sterilize at 110 to 115°C. 

 Reference: Linde (1913 p. 372), Zikes 



(1915 p. 530), Besson (1920 p. 30). 



1350. Stutzer's Nitrate Meat Extract 

 Solution 

 Constituents: 



1. Water 



2. KNO3 



3. Liebig's Meat Extract 

 Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Adjust the reaction to a slight 

 alkalinity. 



(3) Distribute in 10.0 cc. lots. 

 Sterilization: Method not given. 



Use: To study denitrification by B. agilis, 

 B. niirovorus, B. Stuizeri, B. Hartlebi. 

 Variants : 



(a) The author used Schiilke and Meyer 

 or Cibils meat extract instead of 

 Liebig's. 



(b) Tanner dissolved 10.0 g. c.p. NaNOa 

 and 3.0 g. of Liebig's meat extract in 

 1000.0 cc. of water. 



Reference: Stutzer (1901 p. 83), Tanner 

 (1919 p. 45). 



1351. Zikes' Basal Glucose Meat Extract 

 Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Meat extract (0.5%) 5.0 g. 



3. Glucose (0.25%) 2.5 g. 



Preparation : 



(1) Dissolve 2, 3 and one of the added 

 nutrients in 1. 



(2) Distribute as desired. 

 Sterilization: Not specified. 



Use: Author used the medium to study the 

 nitrogen sources suited to the development 

 of Cladothrix dichotoma and Cladothrix 

 natans. He reported that Cladothrix 

 dichotoma grew well on any nitrogen 

 source but Cladothrix natans grew only 

 when peptone or asparagin was added. 

 Bloch studied the nitrogen sources suited 

 for the development of Zoogloea rami- 

 gera. He reported that asparagin pep- 

 tone, ammonium sulphate and then 

 nitrate was the order of the materials as a 

 nitrogen source for Zoogloea ramigera. 



Added nutrients: Zikes as well as Bloch 

 added 0.25% of one of the following: 

 (NH 4)280 4 asparagin 



KNO3 peptone 



Reference: Zikes (1915 p. 542), Bloch (1918 

 p. 51). 



1352. Stutzer's Glucose Meat Extract 

 Solution 



Constituents : 



1. Water. 



2. Glucose. 



3. KNO3 



4. Liebig's ]\Ieat Extract. 

 Preparation : 



(1) Dissolve 2, 3 and 4 in 1. (Peptone 

 may be added). 



(2) Reaction to be slightly alkaline. 



(3) Distribute in 10.0 cc. lots. 

 Sterilization: Method not given. 



Use: To study denitrification by B. agilis, 

 B. niirovorus, B. Stutzeri, B. Hartlebi. 

 Variants : 



(a) The author substituted Schiilke and 

 Meyer's meat extract or Cibils meat 

 extract for Liebig's. 



