CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



397 



(b) Kappen studied nitrate decomposi- 

 tion by cyanamide decomposing 

 bacteria, in a medium composed of 

 5.0 g. meat extract, 2.0 g. glucose and 

 2.0 g. KNO3 per liter. 

 Reference: Stutzer (1901 p. 83), Kappen 



(1909 p. 395). 



1353. Hurler's Succinate Meat Extract 



Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Meat e.xtract, Cibils 100.0 to 200.0 cc. 



3. Ammonium succi- 

 nate 10.0 g. 



Preparation : 



(1) Thicken 100 to 200.0 cc. of Cibils 

 meat extract on the water bath. 



(2) Transfer the viscous mass to a cru- 

 cible and carbonize and ash under the 

 hood. 



(3) Take 3.0 g. of the grey white ash thus 

 obtained and dissolve in 1 liter of 

 water by boiling an hour. 



(4) Filter repeatedly thru cotton. 



(5) Add 10.0 g. of ammonium succinate 

 and neutralize with dilute ammonia. 



(6) Distribute the slightly opalescent 

 medium in tubes. 



Sterilization: Sterilize (method not given). 

 The medium becomes clear. 



Use: Cultivation of typhoid and inter- 

 mediate group. 



Reference: Hurler (1912 p. 356). 



1354. Bloch's Basal Asparagin Meat Extract 



Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Meat extract (1.0%) 10.0 g. 



3. Asparagin (0.25%) 2.5 g. 



Preparation: 



(1) Dissolve 2, 3 and 0.25% of one of the 

 added nutrients in 1. 



(2) Tube or flask. 

 Sterilization: Not specified. 



Use: Cultivation of Zoogloea ramigera. 

 Author reported that growth was gener- 

 ally better without carbohydrates. 

 Added nutrients: The author added 0.25% 

 of one of the following: 



d-glucose lactose 



d-levulose starch 



d-mannose raffinose 



1355. Kappen's Urea Meat Extract Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Meat extract (1.0%) 10.0 g. 



3. Urea (5.0%) 50.0 g. 



Preparation: 



(1) Dissolve 5.0% urea in a 1.0% meat 

 extract solution. 



(2) Distribute in 100.0 cc. lots. 

 Sterilization: Method not given. 



Use: To study decomposition of urea by 

 Urobacillus Pasteuri, Planosarcina ureae, 

 Bacterium Zopfi and Cyanamide decom- 

 posing bacteria. 



Variants: Murray studied ammonification 

 using a 1.0% urea meat infusion solution. 



References: Kappen (1909 p. 394), Murray 

 (1916 p. 598). 



1356. Viehoever's Urea Meat Extract 

 Solution 



Constituents: 



1. Water 



2. K2HPO4. 



3. CaCl2 



4. xMgS04 



5. NaCl 



8. FeCU 



7. Urea (2.0%) 20.0 g. 



8. Liebig's meat extract 



(0.5%) 5.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7 and 8 in 1. 

 Sterilization: Not specified. 

 Use: To study spore production by Urea 



splitting organisms, Bac. probatvs. 

 Reference: Viehoever (1913 p. 214). 



1357. Kappen's Cyanamide Meat^Extract 

 Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Meat extract (1.0%) 10.0 g. 



3. Cyanamide (0.5%) 5.0 g. 



Preparation: 



(1) Prepare a 1.0% meat extract solution. 



(2) Make slightly alkaline to litmus by 

 the addition of Na2C03. 



