398 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(3) Add 0.5% Cyanamide to (2). 



(4) Distribute in Erlenmeyer flasks. 

 Sterilization: Method not given. 



Use: To study cyanamide decomposition 



by bacteria. 

 Reference: Kappen (1909 p. 392). 



1358. Homer's Tryptophane Gelatin 

 Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Nutrient salts 



3. Tryptophane (0.15%) 1.5 g. 



4. Gelatin (1.0%) 10.0 g. 



Preparation : 



(1) Add 1.0% gelatin and 0.15%, tryopto- 

 phane to a solution containing neces- 

 sary nutrient salts. 

 Sterilization: Not specified. 

 Use: Indol production. 

 Variants: The author added 1.0% glucose. 

 Reference: Homer (1906 p. 402). 



1359. Remy and Rosing's Gelatin Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Gelatin (0.8%) 8.0 g. 



Preparation : 



(1) Add 0.8% gelatin to 1000.0 cc. water. 



(2) Neutralize with soda. 



(3) Distribute into small Erlenmeyer 

 flasks. 



Sterilization: Sterilize in streaming steam 

 by means of the fractional method. 



Use: To study decomposition of organic N 

 materials by soil forms. 



Reference: Remy and Rosing (1911 p. 39). 



1360. Standfuss and Kallert's Bone Jelly 



Solution 



Constituents : 



1. Water. 



2. Bone jelly. 

 Preparation : 



(1) Bone jelly is obtained from a factory 

 where fresh bones are rendered under 

 a pressure of 2 to 4 atmospheres in an 

 autoclave. The rendered material 

 is evaporated or condensed to a jelly- 

 like material which is quite stable. 



(2) Dissolve some of the jelly in hot 

 water. The amount depends on the 

 consistency of the jelly. 



(3) Clarify and filter, obtaining a com- 

 pletely clear golden yellow fluid. 



(4) To obtain best results it is well to add 

 some albuminous material. Peptone 

 may be added but a material from the 

 bone jelly gives equally good results. 

 Mix equal parts of water, bone jelly 

 and HCl (strength not specified) and 

 heat in a water bath for 24 hours. 

 Neutralize with soda, clarify and 

 filter. 



(5) Add 1.0% of (4) to (3). 

 Sterilization: Not specified. 



Use: Ine.xpensive culture medium. 

 Reference : Standfuss and Kallert (1920-21 

 p. 223). 



1361. Berman and Rettger's Gelatin 

 Solution 

 Constituents: 



1. Water 1000.0 cc. 



2. Gelatin 2.5 g. 



3. NaCl 5.0 g. 



Preparation : 



(1) Dissolve 2 and 3 in 1. (2.5 g. Liebig's 

 meat extract may be added.) 



(2) Tube in 10.0 cc. lots. 

 Sterilization: Not specified. 

 Use: To study bacterial nutrition. 

 Reference: Berman and Rettger (1918 p. 



381). 



1362. Fleming's Blood and Minced Meat 



Medium 

 Constituents: 



1. Meat 



2. Blood. 

 Preparation : 



(1) Add a little blood to a minced meat 

 medium such as is commonly used to 

 grow anaerobes. 

 Sterilization: Not specified. 

 Use: Preservation of B. influenzae. 



Author reported that subcultures from 



this medium after 6 weeks gave growths as 



copious as after two days. 

 Variants: Harvey added a little whole 



blood to the meat medium as prepared in 



medium 1239. 

 References: Fleming (1919 p. 139), Harvey 



(1921-22 p. 72). 



1363. Dean and Nouat's Blood Egg Medium 



(Cutler) 

 Constituents: 



1. Water 300.0 cc. 



2. Egg 1 



3. Blood, human drop 



