CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



401 



1369. Bacto Egg— Meat Medium 



(Dehydrated) 

 Constituents : 



1. Water 1000-0 cc. 



2. Egg albumin 



3. Beef 1-0 lb. 



4. CaCOa : 5.0 g. 



Preparation: 



(1) Suspend 150.0 g. of Bacto Egg-Meat 

 Medium (Dehydrated) in 1000.0 cc. 

 of distilled water. (Each liter con- 

 tains the whites of 6 eggs, 1 pound of 

 lean beef and 5.0 g. CaCOs). 



(2) Distribute an even mixture in tubes. 



(3) If sterilized at 15 pounds pressure for 

 20 minutes, pH = 7.2 ±. 



Sterilization: Sterilize 20 minutes at 15 

 pounds, allowing autoclave to lose pres- 

 sure slowly. 



Use: Cultivation of anaerobes. 



Reference: Digestive Ferments Co. (1925 

 p. 11). 



1370. Rettger's Egg-Meat Medium 



1000.0 cc. 

 1.0 lb. 



5.0 



Constituents: 



1. Water 



2. Beef 



3. Egg white 



4. CaCOa (0.5%) 



Preparation: 



(1) Thoroly mix 1 pound of lean chopped 

 beef with 500.0 cc. of water, 



(2) Neutralize (1) with NasCOs. 



(3) Heat in the Arnold sterilizer for 30 

 minutes, stirring occasionally. 



(4) Allow to stand for several hours and 

 remove the fatty scum. 



(5) Mix the whites of 6 eggs with 500.0 

 cc. of water. 



(6) Neutralize (5). (Method not given). 



(7) Heat in the Arnold for 30 minutes 

 with occasional stirring to coagulate. 



(8) Mix (4) and (7) and place into a liter 

 flask. 



(9) Add 0.5% of powdered calcium car- 

 bonate to (8). 



Sterilization: Sterilize at 110 to 112°C. 

 in the autoclave for 30 minutes. 



Use: To study putrefaction by B. coli 

 communis, B. lactic aerogenes, B. putri- 

 ficus, bacillus of malignant oedema, 

 bacillus of symptomatic anthrax. The 

 author replaced the air in the flask with 

 hydrogen gas. 



References: Rettger (1907 p. 75), Tanner 

 (1919 p. 58). 



1371. Besredka's Egg Meat Infusion 



Medium (Bezangon) 



Constituents: 



1. Meat infusion solution 1000.0 cc. 



2. Egg. 

 Preparation: 



(1) Prepare a meat infusion solution (no 

 peptone or NaCl added). 



(2) To each 100.0 cc. of sterile (1) con- 

 tained in Roux flasks add 20.0 cc. of a 

 sterile 10.0% egg white solution and 

 from 5.0 to 20.0 cc. of a sterile 10.0%, 

 egg yolk solution under aseptic 

 conditions. 



Sterilization: Method not given. The in- 

 fusion, egg white and egg yolk solutions 

 are sterilized separately. 



Use: Cultivation of tubercle bacilli. 



Reference: Bezangon (1920 p. 546). 



1372. Picker's Sputum Serum Solution 



Constituents: 



1. Serum (beef) 200.0 cc. 



2. Sputum 100.0 cc. 



3. Glycerol (2.0%) 6.0 g. 



Preparation: 



(1) Mix two parts sterile beef serum with 

 one part sterile tuberculous or bron- 

 chitus sputa. 



(2) Add 2.0% glycerolto (1). 

 Sterilization: Sterilize tuberculous or bron- 



chitus sputa for an hour on 3 successive 

 days in streaming steam. Method of 

 sterilization of serum or glycerol not 

 given. 



Use: Cultivation of tubercle bacilli. 



Reference: Ficker (1900 p. 508). 



1373. Kayser's Blood Bile Solution 



Constituents : 



1. Bile, beef 5.0 cc. 



2. Blood.., 2.5 cc. 



Preparation: 



(1) Place 5.0 cc. of sterile beef bile into a 

 sterile test tube. 



(2) Add about 2.5 cc. of the patient's 

 blood that has been removed from a 

 finger tip or an ear under aseptic 

 conditions. 



Sterilization: Method of sterilization of 

 bile not specified. 



