402 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Use: Enrichment medium for typhoid and 

 paratyphoid used in diagnosis by blood 

 culture method, 



Reference: Kayser (1906 p. 186), Klimmer 

 (1923 p. 214). 



1374. Noguchi's Blood Serum Solution 



Constituents: 



1. Serum...., 100.0 cc. 



2. Ringer's Solution 300.0 cc. 



3. Plasma, citrated, rabbit 150.0 cc. 



Preparation : 



(1) Mix 1 part rabbit serum with 3 parts 

 Ringer's solution (see medium 180) 

 and 0.5 part of citrated rabbit plasma. 



Sterilization: Not specified. 



Use: Cultivation of Spirochaeta ictero- 

 haemorrhagiae, Leptospira icterohaemor- 

 rhagiae and other spirochaeteceae. The 

 medium was covered with a layer of 

 paraffin oil following inoculation with 

 suspected blood material. 



Variants : 



(a) The author used 0.9% NaCl solution 

 instead of Ringer's solution. 



(b) The author cultivated Leptospira 

 icterohaemorrhagiae on a mixture of 1.5 

 parts rabbit serum, 4.5 parts Ringer's 

 solution and 1.0 part citrated plasma. 



(c) Kaneko cultivated Spirochaeta ic- 

 terohaemorrhagiae and Spirochaeta 

 hebdomadis on medium prepared as 

 follows: 



(1) Introduce a drop of dog blood into 

 2.0 to 3.0 cc. of dog serum diluted 

 with 1 to 5 parts Ringer solution. 



(2) Heat for 30 minutes at 56 to 58°C. 



(3) Cover with liquid sterile paraffin. 

 References: Noguchi (1917 p. 761), (1918 p. 



606), Kaneko (1921-22 p. 354), Abbott 

 (1921 p. 635). 



1375. Berman and Rettger's Casein Meat 

 Extract Medium 



Constituents : 



1. Water 1000.0 cc. 



2. Casein 1.25 g. 



3. Liebig's meat extract 5.0 g. 



4. NaCl... 5.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Tube in 10.0 cc. quantities. 

 Sterilization: Not specified. 



Use: To study bacterial nutrition. 



Reference: Berman and Rettger (1918 p. 



382). 



1376. Adams' Coke Milk Medium 



Constituents: 



1. Milk. 



2. Coke. 

 Preparation : 



(1) Add sterile pieces of coke to skimmed 

 milk. 



Sterilization: Method not given in the 

 abstract. 



Use: Cultivation of butyric acid bacilli. 

 Before use the oxygen was removed by 

 heating and after inoculation with the 

 fecal material the tubes were again 

 heated for 2 to 3 minutes at 70°C. to 

 eliminate the less resistant organisms. 



Reference: Adams (1921 p. 59). Taken 

 from (1921 p. 319). 



SUBGROUP I-C. SECTION 17 



Liquid media or basal solutions not 

 containing digests, but containing extracts 

 of ashes, soil or similar materials. 

 Ai. Ashed material employed. 

 Harrison and Barlow's Basal Wood 



Ash Medium . . , 1377 



Pasteur's Sucrose Yeast Ash Solu- 

 tion (Smith) 1378 



A2. Soil or soil constituents employed. 

 Bi. Humic acid or its salts used. 

 Krzemieniewska's Glucose Humate 



Solution (Vogel) 1379 



Sohngen's Salt Humate Solution... . 1380 

 Bj. Soil infusions used. 

 Ci. Containing no additional organic 



nutrients. 

 Di. Inorganic nitrogen added. 

 Gutzeit's Ammonium Sulphate Soil 



Infusion 1381 



Stoklasa's Nitrate Soil Infusion I . . 1382 

 Stoklasa's Nitrate Soil Infusion II.. . 1383 

 D2. Inorganic nitrogen not added. 

 Lohnis and Pillai's Basal Soil In- 

 fusion 1384 



Buchanan's Basal Soil Infusion 1385 



Saltet's Sulphite Mud Solution 1386 



Lohnis' Soil Infusion Medium 1387 



Zikes' Soil Infusion Medium 1388 



Harvey's Soil Infusion Medium 1389 



C2. Containing additional organic nutrients 

 Di. Organic nitrogen added. 

 Lohnis Urea Soil Infusion Medium.. 1390 



