404 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



References: Harrison and Barlow (1907 

 pp. 267, 269), Percival (1920 p. 204), 

 Giltner (1921p. 377). 



1378. Pasteur's Sucrose Yeast Ash Solution 



(Smith) 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Ammonium tartrate 10.0 g. 



3. Yeast ash 10.0 g. 



4. Sucrose 100.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1 in the cold. 

 Sterilization: Not specified. 

 Use: Cultivation of yeast. 

 Variants : 



(a) Roux and Rochaix, and Bezangon, 

 used 0.75 g. yeast ash and omitted 

 the ammonium tartrate. 



(b) Kolle and Wassermann used the ashes 

 from 10.0 g. yeast. 



(c) Tanner, Besson, Bezangon, Dopter 

 and Sacquepee, used 1.0 g. ammo- 

 nium tartrate and used 0.75 g. yeast 

 ashes. 



References: Smith (1905 p. 197), Roux and 

 Rochaix (1911 p. 103), Kolle and Wasser- 

 mann (1912 p. 393), Tanner (1919 p. 66), 

 Besson (1920 p. 36), Bezangon (1920 p. 

 115) Dopter and Sacquepee (1921 p. 

 121), Harvey (1921-22 p. 120). 



1379. Krzemieniewska's Glucose Humate 



Solution (Vogel) 



Constituents : 



1. Water 1000.0 cc. 



2. MgS04-7H20 0.125 g. 



3. CaHP04-2H20, 0.25 g. 



4. Sodium humate 10.0 cc. 



5. Glucose 9.0 g. 



Preparation: 



(1) Prepare a sodium humate solution 

 by shaking 1000.0 g. of humus soil 

 in a dilute solution of NaOH. 



(2) Filter thru glass wool. 



(3) Acidify the filtrate with HCl. 



(4) Wash the precipitate until the wash 

 water shows a very weak acid 

 reaction. 



(5) Dissolve the filtrate in weak NaOH 

 solution. 



(6) Dissolve 2, 3 and 5 in 1. 



(7) Add 10.0 cc. of (5) to (6). 

 Sterilization: Not specified. 



Use: To study nitrogen assimilation by 

 azotobacter. Vogel reported little differ- 

 ence in amount of nitrogen assimilated 

 whether K2SO4 be present or not. Pos- 

 sibly there was slightly more nitrogen 

 assimilated per mg. of glucose utilized if 

 the potassium salt was not added. 



Variants: The author added 0.25 g. K.SO^ 

 to the medium. 



Reference: Vogel (1911-12 p. 419). 



1380. Sohngen's Salt Humate Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. MgS04 0.5 g. 



3. Bipotassium phosphate 0.5 g. 



4. NH4CI 0.5 g. 



5. Sodium humate 1-0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not specified. 

 Use: Cultivation of paraffin and petroleum 



oxidizers, Mycobacterium, B. fluorescens 



liquefaciens and Micrococcus paraffinae. 



Author reported that sodium humate was 



utilized by these organisms. 

 Reference: Sohngen (1913 p. 603). 



1381. Gutzeit's Ammonium Sulphate Soil 

 Infusion 



Constituents : 



1. Water.. 1000.0 cc. 



2. Soil 



3. (NH4)2S04 (1.0%) 10.0 g. 



4. CaCOs (0.5%) 5.0 g. 



Preparation : 



(1) Prepare soil extract. 



(2) Add 1.0% (NH4)2S04 and 0.5% 

 CaCOsto (1). 



Sterilization: Not specified. 

 Use: To study denitrification. 

 Variants: Lohnis prepared a similar me- 

 dium as follows: 



(1) Heat 1000.0 g. of good rich garden 

 soil with a liter of tap water for 30 

 minutes in the autoclave under pres- 

 sure of 1 atmosphere or boil with 2 

 liters of water over a free flame. 



(2) Pour off the turbid liquid. 



(3) Mix talc with the liquid. 



(4) Filter thru a double filter paper. 



(5) Make up the volume to 800.0 cc. if 

 necessary. 



