CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



405 



(6) Dissolve 1.0% (NH 4)2804 and 0.5% 

 K2HPO4 in (5). 



(7) Add chalk after inoculation with soil. 



(8) Sterilization not specified. 

 Reference: Gutzeit (1906 p. 370), Lohnis 



(1913 p. 110). 



1382. Stoklasa's Nitrate Soil Infusion I 



Constituents: 



1. Distilled water 



2. Soil 9000.0 g. 



3. NaNO, 1-5 g. 



4. K2HPO4 10 g- 



5. MgSOi 10 g- 



Preparation : 



(1) Extract 9000.0 g. of soil with distilled 

 water at 60 °C. (Amount of water or 

 time not specified.) 



(2) Concentrate the extract (19 liters) 

 to 1 liter. This liter contains 2.2 g. of 

 carbon in the form of soluble organic 

 compounds. 



(3) Add 1.5 g. NaNOa, 1.0 g. KjHPO* 

 and 1.0 g. MgS04 to 600.0 cc. of (2). 



Sterilization: Sterilize four times in the 

 steamer. 



Use: To study denitrification by Bad. 

 Hartlebi. Author reported no deni- 

 trification. 



Reference: Stoklasa (1907 p. 32). 



1383. Stoklasa's Nitrate Soil Infusion II 



Constituents : 



1. Distilled water 



2. Soil 2500.0 g. 



3. NaNOs 0.5 g. 



4. CaCOs 2.5 g. 



Preparation : 



(1) Extract 2500.0 g. soil with distilled 

 water three times (15 liters of water in 

 all). 



(2) Evaporate to 160.0 cc. This extract 

 contains 2.55 g. organic carbon per 

 liter (determined by oxidation with 

 chromic acid). 



(3) Add 0.5 g. NaNOs, containing 0.08 

 g. N and 2.5 g. CaCOa to 150.0 cc. 

 of (2). 



Sterilization: Sterilize four times in /the 

 steamer. 



Use: To study denitrification by Bad. 

 Hartlebi. Author reported no deni- 

 trification. 



Reference: Stoklasa (1907 p. 33). 



1384. Lohnis and Pillai's Basal Soil Infusion 



Constituents : 



1. Water 1000.0 cc. 



2. Soil 1000.0 g. 



3. K2HPO4 (0.5%) 5.0 g. 



4. CaCOs (1.0 to 2.0%). 10.0 to 20.0 g. 

 Preparation : 



(1) Add 1000.0 g. soil to 1 liter of water. 



(2) Place in a covered pot and autoclave 

 for 30 minutes at 1 atmosphere pres- 

 sure. 



(3) Filter. One obtains about 600.0 cc. 



(4) To (3) add 0.5% K2HPO4 and 1.0% 

 of the added nutrients. 



(5) Add 1.0 to 2.0% CaCO,. 

 Sterilization: Not specified. 



Use: To study N assimilation. Author 

 reported that more nitrogen was assim- 

 ilated with mannitol than glucose, 

 CaCOa favored the nitrogen assimilation 

 with sugar but not with the acids. 

 Variants: The author omitted the CaCOs. 

 Added nutrients: The author added 1.0% 

 of one of the following: 

 mannitol 

 glucose 



tartaric acid (neutralized with soda) 

 Reference: Lohnis and Pillai (1907 p. 88). 



1385. Buchanan's Basal Soil Infusion 

 Constituents : 



1. Water 1000.0 cc. 



2. Soil 100.0 g. 



Preparation : 



(1) Boil 100.0 g. of rich garden soil with 1 

 liter of water. 



(2) Filter until clear. 



(3) Dissolve 2.0% of one of the added 

 nutrients in (2). 



Sterilization: Sterilize for 20 minutes on 

 each of 3 successive days in streaming 

 steam. 

 Use: To study gum production. 

 Added nutrients and variants: The author 

 added 2.0% of one of the following: 

 glucose sucrose 



levulose lactose 



galactose mannitol 



maltose 

 Reference: Buchanan (1909 p. 388). 



1386. Saltet's Sulphite Mud Solution 

 Constituents : 

 1. Water (ditch). 



