406 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



2. Mud. 



3. NasSO,. 

 Preparation : 



(1) Mix ditch water and mud. 



(2) Distribute in test tubes. 



(3) To each tube of sterile (2) add two 

 drops of a 10.0% NajSOs solution. 



Sterilization: Sterilize (2) at 110°C. 



Use: To study sulphite reduction by B. 



desulfuricans . 

 Variants : The author added two drops of a 



10.0% Na2S20j solution to each tube 



instead of NajSOa- 

 Reference: Saltet (1900 p. 697). 



1387. Lohnis' Soil Infusion Me4ium 



Constituents : 



1. Water (tap) 1000.0 cc. 



2. Soil 1000.0 g. 



Preparation : 



(1) Heat 1000.0 g. of good rich garden 

 soil with a liter of tap water for 30 

 minutes in the autoclave under pres- 

 sure of 1 atmosphere or boil with 2 

 liters of water over a free flame. 



(2) Pour off the turbid liquid. 



(3) Mix talc with the liquid. 



(4) Filter thru a double filter paper. 



(5) Make up the volume to 800.0 cc. if 

 necessary. 



Sterilization: Method not given. 



Use: Cultivation of soil microorganisms. 



Variants : 



(a) Sherman boiled one part soil with 3 

 parts water, filtered until clear and 

 then added a small excess of CaCOs. 

 He reported that this medium was 

 especially adapted for the cultivation 

 of flagellates, ciliates and amoebae. 



(b) Giltner used 2 liters of water to 1000.0 

 g. soil. 



References: Lohnis (1913 p. 101), Sherman 

 (1916 p. 42), Giltner (1921 p. 370), Harvey 

 (1921-22 p. 105), Cunningham (1924 p. 

 136). 



1388. Zikes' Soil Infusion Medium 



Constituents : 



1. Water 1000.0 cc. 



2. Soil 100.0 g. 



3. KH2PO4 (0.3%) 3.0 g. 



4. MgS04 (0.2%) 2.0 g. 



Preparation : 



(1) Extract 100.0 g. of soil with 1000.0 

 cc. water by boiling. 



(2) Filter. 



(3) Add 0.3% KH2PO4 and 0.2% MgSOi- 

 Sterilization: Not specified. 



Use: Cultivation of Apiculatus yeast, To- 

 rula alba, Torvla Molischiana, Myco- 

 derma cerevisiae Blasioderma salmoni- 

 color. 



Reference: Zikes (1911 p. 147). 



1389. Harvey's Soil Infusion Medium 



Constituents: 



1. Water (tap) 1000.0 cc. 



2. Soil lOC.O g. 



3. K2HPO4 0.5 g. 



Preparation : 



(1) Boil 100.0 g. of rich garden soil in 

 200.0 cc. of water. 



(2) Pour ofT the turbid liquid and add a 

 little talc. 



(3) Filter until clear thru thick filter 

 paper. 



(4) Add 900.0 cc. of tap water and 0.5 g. 

 K2HPO4 to 100.0 cc. of (3). 



Sterilization: Not specified. 



Use: Cultivation of flagellates and ciliates 



from the soil. 

 Reference: Harvey (1921-22 p. 108). 



1390. Lohnis Urea Soil Infusion Medium 



Constituents : 



1. Water 1000.0 cc. 



2. Soil 1000.0 g. 



3. K2HPO4 (0.05%) 0.5 g. 



4. Urea (5.0%,) 40.0 g. 



Preparation : 



(1) Prepare soil infusion as indicated in 

 medium 1387. 



(2) Dissolve 0.05% K2HPO4 and 5.0% 

 urea in (1). 



Sterilization: Not specified. 



Use: Cultivation of organisms capable of 



utilizing urea. 

 Reference: Lohnis (1913 p. 108), Giltner 



(1921 p. 374). 



1391. Lohnis and Moll's Asparagln Soil 

 Infusion Medium 



Constituents: 



1. Water 1000.0 cc. 



2. Soil 500.0 g. 



3. K2HPO4 (0.5%) 5.0 g. 



4. Glucose (0.1%) '. . 1.0 g. 



5. Asparagin (0.1%) 1.0 g. 



