CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



407 



Preparation: 



(1) Allow 50.0% soil to stand in water for 

 20 hours and then filter. 



(2) Dissolve 2, 3 and 4 in (1). 

 Sterilization: Medium may or may not be 



sterilized. 

 Use: Cultivation of organisms capable of 



utilizing calcium cyanamide. 

 Variants : 



(a) The authors added 0.014 g. of calcium 

 cyanamide. 



(b) Lohnis prepared the medium by 

 dissolving 0.2% calcium cyanamide, 

 0.01% glucose, 0.05% K2HPO4 and 

 0.01% asparagin in soil infusion pre- 

 pared as in medium 1387. 



References: Lohnis and Moll (1907 p. 265), 

 Lohnis (1913 p. 108). 



1392. Perotti's Dicyandiamid Soil Infusion 

 Medium 



Constituents: 



1. Water 2000.0 cc. 



2. Garden soil 1000.0 g. 



3. Dicyandiamid 2.0 g. 



4. K2HPO4 0.5 g. 



5. Glucose 0.1 g. 



Preparation : 



(1) Boil 1000.0 g. garden soil with 200.0 

 cc. of water for 2 hours. 



(2) Concentrate the liquid a little and 

 filter. 



(3) Dilute until the filtrate contains 

 0.4% mineral substances. 



(4) Adds, 4 and 5 to (3). 

 Sterilization: Not specified. 



Use: Cultivation of organisms utilizing 



dicyandiamid from the soil. 

 Reference: Perotti (1908 p. 207). 



1393. Gutzeit's Mannitol Soil Infusion 

 Medium 

 Constituents : 



1. Water 1000.0 cc. 



2. Soil 



3. Mannitol (1.0%) 10.0 g. 



Preparation : 



(1) Dissolve 1.0% mannitol in a soil 

 infusion. 



Sterilization: Not specified. 



Use: Gutzeit used the medium to study 

 denitrification. Lohnis and Cunning- 

 ham used similar media for the enrich- 

 ment of nodule bacteria and azotobacter. 



Variants: Lohnis added 1.0% mannitol and 

 0.05% K2HPO4 to soil infusion (see 

 medium 1387) and sterilized in the auto- 

 clave. 



References: Gutzeit (1906 p. 370), Lohnis 

 (1913 p. 112), Cunningham (1924 p. 155). 



1394. Jensen's Nitrate Soil Infusion 

 Medium 

 Constituents : 



1. Water 1000.0 cc. 



2. Soil 500.0 g. 



3. NaNOa 20.0 g. 



4. Glycerol 

 Preparation: 



(1) Add 1000.0 cc. of 2.0% solution of 

 NaNOs to 500.0 g. of soil that has 

 been passed thru a 0.5 mm. sieve. 



(2) Add 0.0, 5.0, 20.0 or 50.0 g. of glycerol 

 to(l). 



Sterilization: Not specified. 



Use: To study denitrification by soil forms. 

 Author reported that the addition of 

 glycerol aided denitrification. No deni- 

 trification occurred without glycerol at 

 the end of 14 days. 



Reference: Jensen (1897 p. 690). 



