412 



CULTURE MEDIA FOR CULTIVATION OF MICROROGANISMS 



Added nutrients: Gordon added various 

 nitrogen compounds. The compounds 

 used or concentration not specified. 



Reference: Gordon (1917 p. 371) taken from 

 (1917 p. 299). 



1405. Carrel and Burrows' Locke's Solution 



Agar 



Constituents: 



1, Locke's solution 1000.0 cc. 



' 2. Agar (2.0%) 20.0 g. 



Preparation: (1) Dissolve 2.0% agar in 



Locke's solution (see medium 6 for 



Locke's solution). 

 Sterilization: Not specified. 

 Use: Cultivation of tissue in vitrio. 



Author reported that the medium did not 



support growth nearly as well as did 



plasma. 

 Reference: Carrel and Burrows (1911 



p. 245). 



1406. Omeliansky's Basal Carbonate Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Agar 15.0 g. 



3. NaoCOs 1.0 g. 



4. K2HPO4 trace 



Preparation: (1) Dissolve 2, 3, 4 and one of 



the added nutrients in 1. 

 Sterilization: Not specified. 

 Use: To study oxidation of sulphites and 



phosphites. The author reported that 



neither the phosphite nor sulphite were 



oxidized. 

 Added nutrients: The author added 0.3 g. 



of one of the following: 



KNO2, KNO3, Peptone. 

 Variants: The author added 0.0 or 2.0 g. 



of NaoSOa or NaaHPOj. 

 Reference: Omeliansky (1902 p. 64). 



1407. Ashby's Salt Agar (Murray) 



Constituents : 



1. Water 1000.0 cc. 



2. K2HPO4 0.2 g. 



3. MgS04 0.2 g. 



4. NaCl 0.2 g. 



5. CaS04 0.2 g. 



6. CaCOj 5.0 g. 



7. Agar 15.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6 and 7 



in 1. 

 Sterilization: Not specified. 



Use: To study nitrogen fixation and to 

 determine number of organisms in soil. 

 Used both anaerobically and aerobically. 



Reference: Murray (1916 p. 608). 



1408. Winogradski's Salt Agar (Murray) 

 Constituents: 



1. Water 1000.0 cc. 



2. K.HPO4 1.0 g. 



3. MgS04 3.0 g. 



4. NaCl 0.01 g. 



5. MnS04 0.01 g. 



6. CaCO, 10.0 g. 



7. FeCIa (10% soln.) 2 drops 



8. Agar 15.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6, 7 



and 8 in 1. 



Sterilization: Not specified. 



Use: lo study nitrogen fixation and to 

 determine the number of bacteria in the 

 soil. Used anaerobically and aerobi- 

 cally. 



Reference: Murray (1916 p. 608). 



1409. Liot's Basal Salt Agar 

 Constituents: 



1. Double distilled water 1000.0 cc. 



2. Sodium phosphate 5.0 g. 



3. MgS04 2.5 g. 



4. Agar 25.0 g. 



Preparation : 



(1) Macerate 25.0 g. of commercial 

 agar in 750.0 cc. of double distilled 

 water for 48 hours. 



(2) Dissolve in the autoclave at about 

 100°C. 



(3) Filter while hot on a Chardin filter 

 paper. 



(4) Dissolve 5.0 g. sodium phosphate in 

 125.0 cc. of double distilled water. 



(5) Dissolve 2.5 g. MgS04 in 125.0 cc. 

 double distilled water. 



(6) Add sterile (4) to (3). Mix well. 



(7) Add sterile (5) to (6). Mix well. 



(8) Tube in 10.0 cc. quantities. 



(9) When ready for use add 0.5 cc. of a 

 1.0% sterile solution of one of the 

 added nutrients to each sterile tube. 

 (The sodium phosphate and MgS04 

 may be omitted, leaving a non- 

 mineral agar.) 



Sterilization: Sterilize (4) and (5) at 115° 

 for 15 minutes. Sterilize (8) in the usual 

 manner. (Method not given.) Method of 



