CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



413 



sterilization of the added nutrients not 

 given. 

 Use: To study pigment production by 



pyocyanius bacilli. 

 Added nutrients: The author added 0.5 cc. 

 of a 1.0% solution of one of the following 

 to each 10.0 cc. of the medium: 

 Ammonia salts of organic acids 



Amides 



Monobasic acids 



Polybasic acids 



Amines 

 Ammonia salts of inorganic acids 



Polyatomic alcohols 



Monosaccharides 



Polysaccharides 



Monoses 

 Reference: Liot (1923 p. 238). 



1410. Dox's Salt Agar (Tanner) 



Constituents : 



1. Distilled water 3000.0 cc. 



2. MgS04 1-^ g- 



3. K2HPO4 3.0 g. 



4. KCl 1-5 S- 



5. FeS04 0.03g. 



6. Agar to solidify 

 Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Solidify by the addition of agar. 

 Sterilization: Not specified. 



Use: Cultivation of fungi. 

 Reference: Tanner (1919 p. 65). 



SUBGROUP II-B. SECTION 2 



Basal or complete media containing agar 

 with inorganic salts. Nitrogen supplied 

 as ammonium salts. 



Ai. Ammonia present as ammonium chlo- 

 ride. 



Beijerinck's Thiosulphate Ammo- 

 nium Chloride Agar 1411 



Waksman's Thiosulphate Ammo- 

 nium Chloride Agar 1412 



Beijerinck and van Delden's Ammo- 

 nium Chloride Agar 1413 



A2. Ammonia present as ammonium 

 nitrate. 



Munter's Basal Ammonium Agar. . . 1414 

 Beijerinck's Ammonium Nitrate 



Agar .--"IS 



A3. Ammonia present as ammonium 

 sulphate. 



Pesch's Basal Ammonium vSulphate 

 Agar 1416 



1417 



1418 

 1419 



1420 



1421 



1422 

 1423 

 1424 



Fremlin's Ammonium Sulphate Agar. 



Nencki, Sieber and Wynikiwicz's 

 Ammonium Sulphate Agar 



Gowda's Ammonium Sulphate Agar. 



Heinemann's Ammonium Sulphate 

 Agar 



Committee S. A. B. Basal Ammo- 

 nium Phosphate Agar 



A 4. Ammonia present as salts of phos- 

 phoric acid. 



Beijerinck's Sodium Ammonium 

 Phosphate Agar. • - • • 



Stutzer's Ammonium Magnesium 

 Phosphate Agar 



Carper's Basal Ammonium Phos- 

 phate Agar 



1411. Beijerinck's Thiosulphate Ammonium 



Chloride Agar 

 Solidify Beijerinck's Thiosulphate Solu- 

 tion, see medium 76 by the addition of 

 2.0% agar. 



1412. Waksman's Thiosulphate Ammonium 



Chloride Agar 



Constituents: 



1. Distilled water 1000.0 cc. 



2. NaoS203-5H20 5.0 g. 



3. KH2PO4 3.0 g. 



4. NH4CI 0.1 g. 



5. MgCh 0.1 g. 



6. CaCU 0-2-5g- 



7. Agar 20.0_g. 



Preparation: Dissolve 2, 3, 4, 5, 6 and 7 m 1. 



Sterilization: Sterilize at 15 pounds pres- 

 sure for 15 minutes. 



Use: Cultivation and isolation of Th. 

 thiooxidans. Author reported that this 

 organism can grow at pH = 1.0. Maxi- 

 mum pH was between 3.0 and 4.0. 



Reference: Waksman (1922 p. 607). 



1413. Beijerinck and van Delden's Ammo- 

 nium Chloride Agar 



Constituents: 



1. Distilled water 



2. Agar 



3. K2HPO4 



4. NH4CI 



1000.0 cc. 



15.0 g. 



0.1 g. 



0.1 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 



Sterilization: Not specified. 



Use: Isolation of Bacillus oligocarbophilus. 



Author reported that colonies were dry, 



snowv white or rose colored. If the agar 



plate" be inoculated with straw or an 



