414 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



impure culture of the organism, a pure 

 culture may be obtained. 

 Reference: Beijerinck and van Delden 



(1903 p. 37). 



1414. Mijnter's Basal Ammonium Nitrate 

 Agar 



Constituents: 



1. Water 



2. MgS04 



3. NaCl 



4. K2HPO4 



5. NH4NO3 



6. CaCU 



7. FeClj 



1000.0 cc. 



0.5 g. 



0.5 g. 



1.5 g. 



1.5g. 



0.2 g. 



trace 



8. Agar 1.25% 12.5 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7, 8 and one of 

 the added nutrients in 1. 



(2) Make slightly alkaline by the addi- 

 tion of Na2C03. 



Sterilization: Not specified. 

 Use: Cultivation of actinomycetes. The 

 author reported that oxalic acid, tartaric 

 acid and hippuric acid were not suitable 

 carbon sources for the actinomycetes 

 studied. The remaining acids were gen- 

 erally fair carbon sources for most of the 

 actinomycetes studied. 

 Added nutrients: The author added one of 

 the following materials: 



acetic acid aspartic acid 



lactic acid citric acid 



oxalic acid hippuric acid 



succinic acid uric acid 



malic acid humus acid 



Reference: Miinter (1913 p. 371). 



1415. Beijerinck's Ammonium Nitrate Agar 



Constituents: 



1. Distilled water 



2. Agar 



3. NH4NO3 



4. Potassium phosphate.., 



5. MgS04 



6. CaCh 



Preparation : 



(1) Wash agar with distilled water for 

 some time to separate the soluble 

 material. 



(2) Dissolve 3, 4, 5 and 6 in 1. 



(3) Dissolve 2.0% (dry) (1) in (2). 



(4) Pour into plates. 

 Sterilization: Not specified. 



Use: Isolation of Pleurococcus vulgaris 

 (green coating on tree trunks, roofs, 

 fences, etc.). To inoculate mix a little 

 of the covering from a linden tree with 

 water and pour over the plates. Pour 

 off the excess water. Small green colonies 

 appeared after 3 weeks. Munter used 

 medium 26 to study the availability of 

 carbon source for Actinomyces. 



Variants: Munter solidified medium 53 by 

 the addition of agar. 



Reference: Beijerinck (1898 p. 785), 

 Munter (1913 p. 368). 



1416. Pesch's Basal Ammonium Sulphate 

 Agar 



Constituents : 



1- Water 1000.0 cc. 



2. K2HPO4 1.0 g. 



3. MgS04 0.5 g. 



4. NaCl 0.02 g. 



6. FeS04 trace 



6. Ca3(P04)2 trace 



7. (NH4)2S04 1.9 g. 



8- Agar 20.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7 and one of the 

 added nutrients in 1. 



(2) Add Na2C03 until the reaction is 

 slightly alkaline. 



(3) Dissolve 20.0 g. of agar that has been 

 washed free from nitrogen in (2). 



Sterilization: Not specified. 



Use: To study nitrogen and carbon utiliza- 

 tion by the colon-typhoid group. Used 

 also as an enrichment medium for the 

 paratyphoid group. The author re- 

 ported that paratyphoid B and colon 

 organisms showed luxuriant growth. 

 Typhoid and paratyphoid A showed only 

 a little growth even after 48 to 72 hours. 



Added nutrients: The author added one of 

 the following materials: 



Glucose 10.0 g. 



Potassium tartrate 10.0 g. 



Sodium citrate 10.0 g. 



References: Pesch (1921 pp. 98, 100), 

 Klimmer (1923 p. 217). 



1417. Fremlin's Ammonium Sulphate Agar 

 Constituents: 



1. Distilled water 1000.0 cc. 



2. (NH4)2S04 1.0 g. 



3. Potassium phosphate 1-0 g. 



