418 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Winogradsky's Sodium Nitrite Agar 

 (Fraenkel) 1425 



Stutzer's Sodium Nitrite Agar 1426 



Heinemann's Sodium Nitrite Agar. . 1427 

 A2. Nitrogen supplied as nitrates. 



Trautwein's Thiosulphate Nitrate 

 Agar..., 1428 



Beijerinck and van Delden's Nitrate 

 Agar 1429 



Noyes' Ammonium Nitrate agar... 1430 



1425. Winogradsky's Sodium Nitrite Agar 



Constituents: 



1. Water. 1000.0 cc. 



2. NaNOj (Merck) 2.0 g. 



3. Soda (water free) 1.0 g. 



4. Potassium phosphate 



5. Agar agar 15.0 g. 



Preparation: 



(1) Dissolve 2, 3 and 5 in 1. 



(2) Dissolve a knife point of potassium 

 phosphate in (1). 



Sterilization: Not specified. 



Use: To study nitrification by nitrifying 

 organisms. Stutzer and Hartleb used a 

 similar medium for the cultivation of the 

 supposedly foot and mouth disease 

 bacteria. 



Variants : 



(a) Stutzer and Hartleb used a medium 

 of the following composition: 



(1) Water 1000.0 cc. 



(2) Agar 20.0 g. 



(3) Potassium phosphate. . . 1.0 g. 



(4) NasCOs..., 1-0 g- 



(5) NaN02 2.0 g. 



(b) Fraenkel specified the use of 1.0 g. 

 potassium phosphate and used 12.5 g. 

 of agar instead of 15.0 g. 



(c) Omeliansky added a trace of potas- 

 sium phosphate instead of a knife 

 point. 



(d) Wimmer specified the use of 0.5 g. 

 KH2PO4 instead of a knife point of 

 potassium phosphate. 



(e) Smith added 0.05 g. of K2HPO4 in- 

 stead of a knife point of potassium 

 phosphate. 



(f) Lohnis added 0.5 g. K2HPO4 instead 

 of a knife point of potassium phos- 

 phate. 



(g) Percival and Harvey used 0.2 g. of 

 K2HPO4 instead of a knife point of 

 potassium phosphate. 



References: Wlnogradsky (1896 p. 425), 

 Stutzer and Hartleb (1897 p. 404), Fraen- 

 kel (1898 p. 10), Omeliansky (1899 p. 548), 

 Wimmer (1904 p. 139), Smith (1905 

 p. 199), Lohnis (1913 p. 110), Percival 

 (1920 p. 150), Harvey (1921-22 p. 107). 



1426. Stutzer's Sodium Nitrite Agar 



Solidify the variant of Winogradsky's 

 Nitrite Solution as given by Stutzer by the 

 addition of agar. (See variant (a) Med. 1425) 



1427, Heinemann's Sodium Nitrite Agar 



Constituents: 



1. Distilled water 



2. MgS04 



3. FeSOi 



4. NaCl 



5. K2HPO4 



« NasCOa (fused) 



7. NaN02 



8. Agar 



Preparation: 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) Dissolve 20.0 g. of agar in (1). 



Sterilization: Sterilize in the autoclave. 



Use: O.xidation of nitrites with the forma- 

 tion of nitrates. 



Reference: Heinemann (1922 p. 39). 



1428. Trautwein's Thiosulphate Nitrate 

 Agar 



Solidify Trautwein's Thiosulphate Solu- 

 tion (see medium 109) by the addition of 

 2.0% agar. 



1429. Beijerinck and van Delden's Nitrate 

 Agar 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Agar 15.0 g. 



3. K2HPO4 0.1 g. 



4. KNO3 0.1 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Not specified. 



Use: Isolation of Bacillus oligocarbophilus 

 Variants: Groenewege used 0.1% KNO3 

 and 0.05% K2HPO4 and added one of the 

 following materials: 

 sucrose sodium propionate 



levulose sodium formate 



maltose sodium oxalate 



mannitol sodium butyrate 



glucose sodium succinate 



