CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



419 



lactose sodium tartrate 



raffinose sodium malate 



• sodium acetate sodium lactate 

 He reported that lactose, sodium acetate, 

 propionate, formate, oxalate or butyrate 

 were not suitable carbon sources and 

 showed no growth. Remaining materials 

 supported growth. Best growth was ob- 

 tained with sucrose, glucose and levulose. 

 References: Beijerinck and van Delden 

 (1903 p. 37), Groenewege (1913 p. 24), 

 Lohnis (1913 p. 106). 



1430. Noyes' Ammonium Nitrate Agar 

 Constituents: 



1. Water 1000.0 cc. 



2. Agar (best) 15.0 g. 



3. NH4NO3 1.0 g. 



Preparation: (1) Dissolve 2 and 3 in 1. 

 Sterilization: Not specified. 



Use: Cultivation of soil organisms. The 



medium was inoculated with soil. 

 Reference: Noyes (1916 p. 93). 



SUBGROUP II-C 



Agar Media with One or More 

 Constituents Organic 



Key to the Sections of Subgroup II-C 



Ai. Chemical composition of all constit- 

 uents known. 



Bi. Nitrogen inorganic, carbon organic 



Section 1 (Med. 1431-1495) 



B2. Nitrogen organic 



Section 2 (Med. 1496-1529) 



A2. Chemical composition of at least one 

 constituent not definitely known. 



Bi. Containing digests. 



Ci. Containing a commercial digest. 



Di. All additional constituents of known 

 chemical composition 



Section 3 (Med. 1530-1596) 



D2. At least one of the additional constit- 

 uents of unknown chemical composition. 



El. Additional unknown constituent of 

 plant or soil origin 



Section 4 (Med. 1597-1627) 



E2. Additional unknown constituent of 

 animal origin. 



Fi. Not containing extracts or infusions of 

 animal origin. .Sections (Med. 1628-1660) 



F2. Containing extracts or infusion of ani- 

 mal origin. . . .Section 6 (Med. 1661-2028) 



C2. Not containing commercial digests; 

 containing non-commercial digests. 



Section 7 (Med. 2029-2078) 



B2. Not containing digests. 



Ci. Containing unknown constituents of 



plant origin. .Section 8 (Med. 2079-2141) 

 C2. Containing unknown constituents of 



animal origin. .Section 9 (Med. 2142-2192) 

 C3. Containing unknown constituents of 



ash or soil origin 



Section 10 (Med. 2193-2198) 



SUBGROUP II-C. SECTION 1 



Basal or complete media containing agar, 

 with all other constituents of known chem- 

 ical composition; nitrogen inorganic, car- 

 bon organic. 

 Ai.* Nitrogen present as free or atmospheric 



nitrogen only. 

 Bi. Carbon supplied as carbohydrates. 

 Ci. Monosaccharides employed. 

 Di. Inorganic salts not added. 



Milburn's Basal Glucose Agar 1431 



Zipfel's Basal Glucose Agar 1432 



Lantzsch's Basal Nitrate Agar 1433 



D2. Inorganic salts added. 

 El. Containing salts of monovalent cations, 

 only. 



Davis' Glucose Agar 1434 



Gerlach and Vogel's Glucose Phos- 

 phate Agar 1435 



Sullivan's Glucose Agar 1436 



Gage's Glucose Agar 1437 



E2. Containing salts of mono and di-valent 

 cations. 



Kisch's Basal Glucose Agar 1438 



Lipman and Brown's Basal Glucose 



Agar 1439 



Beijerinck's Glucose Agar 1440 



Heinemann's Glucose Agar 1441 



Miinter's Basal Salt Agar 1442 



C2. Disaccharides employed. 

 Groenewege's Basal Sucrose Agar. . 1443 



Owen's Sucrose Agar 1444 



Buchanan's Sucrose Agar 1445 



Lohnis' Congo Red Sucrose Agar. . . 1446 

 C3. Polysaccharides employed. 



Krainsky's Basal Starch Agar 1447 



Wyant and Tweed's Starch Agar. . . 1448 



Welch's Dextrin Agar 1449 



B2.t Carbon supplied as alcohols. 

 Ci. Inorganic salts not added. 



Hesse's Glycerol Agar 1450 



C2. Inorganic salts added. 



* See page 420 for A2 and A3. 

 t See page 420 for B3. 



