CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



421 



Bj. More than one source of organic car- 

 bon added. 



Conn and Breed's Double Sugar Ni- 

 trate Agar 1495 



1431. Milburn's Basal Glucose Agar 



Constituents: 



1. Water 1000.0 cc. 



2. Agar 



3. Glucose (5.0%) 50.0 g. 



Preparation: 



(1) Dissolve 3 and one of the added nu- 

 trients in 1. 



(2) Solidify by the addition of agar. 

 Sterilization: Method not given. 



Use: To study color formation. The 

 author reported that tyrosine and aspara- 

 gin gave concentric circles. Leucin and 

 albumin gave poorly defined green and 

 yellowish white rings. Remaining ma- 

 terials gave no rings. Krainsky used a 

 similar medium for the cultivation of 

 actinomyces. 



Added nutrients: The author added 2.0% 

 of one of the following: 



tyrosine alcumin 



asparagin nuclean 



leucine peptone 



albumin 



Variants: Krainsky used 1.0% glucose and 

 added one of the following materials: 



KNO3 0.05% 



NH4CI 0.05% 



Calcium malate 1 .0% 



Peptone 0.05% 



Asparagin 0.05% 



References: Milburn (1904 p. 136), 

 Krainsky's (1914 p. 662). 



1432. Zipfel's Basal Glucose Agar 



Constituents: 



1. Water 1000.0 cc. 



2. Agar (3.0%) 30.0 g. 



3. Glucose (1.0%,) 10.0 g. 



4. Malic acid 

 Preparation : 



(1) Dissolve 2, 3 and one of the added 

 nutrients in 1. 



(2) Acidify slightly by the addition of 

 malic acid. 



Sterilization: Not specified. 



Use : Cultivation of nodule bacteria. 



Added nutrients: The author added 2.0% 

 of one of the following: 



plasmon sanatogen 



tropon nutrose 



roborat 



Reference: Zipfel (1911 p. 128). 



1433. Lantzsch's Basal Nitrate Agar 

 Constituents: 



1. Water 1000.0 cc. 



2. Agar (2.0%) 20.0 g. 



3. K2HPO4 (0.01%) 0.1 g. 



4. NaN03 (0.01%) 0.1 g. 



5. CaCls (0.005%o) 0.05 g. 



6. MgS04 traces 



7. FeCla trace 



Preparation : 



(1) Wash agar shreds in running water 

 for a week. 



(2) Soak (1) in distilled water for one day. 



(3) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(4) Pour thick plates. 



(5) Incubate the inoculated medium in 

 an atmosphere of one of the added 

 nutrients. 



Sterilization: Not specified. 



Use: Cultivation of Actinomyces oligo- 

 carbophilus (Bacillus oligocarbophilus) . 



Added nutrients: The author incubated the 

 medium in an atmosphere of acetic acid, 

 butyric acid or acetone. The atmos- 

 phere should contain 0.005 g. acetic acid, 

 0.005 g. acetone or 0.004 g. butyric acid 

 per 100.0 cc. atmosphere. 



Reference: Lantzsch (1922 p. 312). 



1434. Davis' Glucose Agar 

 Constituents: 



1. Water 1000.0 cc. 



2. Agar 15.0 g. 



3. NaCl 5.0 g. 



4. Glucose 20.0 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Not specified. 



Use: To study chromogenesis by sporo- 

 tricha. Author reported that if maltose 

 be substituted for glucose, growth and 

 pigment production was more luxuriant. 

 Using glucose, pigment formation was 

 slight. Growth without glucose about 

 the same as when glucose (or impure glu- 

 cose) was employed. 



Reference: Davis (1915 p. 178). 



