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CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



1435. Gerlach and Vogel's Glucose 

 Phosphate Agar 



Constituents: 



1. Water 1000.0 cc. 



2. Agar 20.0 g. 



3. Potassium bi-phosphate. .. . 2.0 g. 



4. Glucose 2.0 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Not given. 



Use: Cultivation of Azotobacter (nitrogen 

 assimilators from the soil). Other in- 

 vestigators used different nitrogen fixing 

 organisms. 



Variants : 



(a) Beijerinck and van Delden used 0.5 g. 

 K2HPO4 instead of 2.0 g. of potassium 

 bi-phosphate, and solidified the me- 

 dium with agar, not specifying the 

 exact amount used. These investi- 

 gators cultivated chroococcum. 



(b) Stoklasa isolated radiobacteria, azo- 

 tobacter and chroococcum in a me- 

 dium composed of 1000.0 cc. of IMol- 

 dau river water, 20.0 g. agar, 20.0 g. 

 glucose and 5.0 g. potassium di- 

 phosphate. 



(c) Percival used 2.0 g. K2HPO4 per liter. 

 References: Gerlach and Vogel (1902 



p. 670), Beijerinck and van Delden 

 (1902 p. 8), Stoklasa (1908 p. 489), Per- 

 cival (1920 p. 182). 



1436. Sullivan's Glucose Agar 



Constituents: 



1. Water 1000.0 cc. 



2. Glucose 20.0 g. 



3. NasPOi 5.0 g. 



4. K2HPO4 5.0 g. 



5. Agar 10.0 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not specified. 



Use: General culture medium. Author re- 

 ported that few organisms were capable 

 of growing in this medium. 



Reference: Sullivan (1905-06 p. 117). 



1437. Gage's Glucose Agar 



Constituents: 



1. Water (nitrate free) 1000.0 cc. 



2. K2HPO4 1.0 g. 



3. NaCl 1.0 g. 



4. Agar 15.0 g. 



5. Glucose 1.0 g. 



Preparation: 



(1) Wash agar thoroly in water until it is 

 free from nitrogen. 



(2) Dissolve 2, 3, 15.0 g. of (1) and 1.0 g. 

 of glucose in 1000.0 cc. of nitrite free 

 water. 



(3) Distribute into large Erlenmeyer 

 flasks. 



Sterilization: Method not given. 



Use: To study nitrogen fixation by nitroso 



bacteria. The author also substituted 



lactose for glucose. 

 Reference: Gage (1910 p. 18). 



1438. Kisch's Basal Glucose Agar 



Medium the same as 129, but solidified by 

 the addition of 2.0% agar. 



1439. Lipman and Brown's Basal Glucose 

 Agar 



Constituents: 



1. Water 1000.0 cc. 



2. Glucose 10.0 g. 



3. K2HPO4 0.5 g. 



4. MgS04 0.2 g. 



5. Agar 20.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and one of the added 

 nutrients in 1. 



(2) Add NaOH to neutralize one-half 

 or one-fourth of the acid or add no 

 NaOH. 



Sterilization: Method not given. 



Use: To determine bacterial count of soils. 

 Other investigators used the basic me- 

 dium without additions for the cultiva- 

 tion of yeast. 



Added nutrients : The authors added 0.05 g. 

 KNO3, 0.04 g. (NH4)2S04 or 0.05 g. pep- 

 tone to the basic solution. 



Variants : 



(a) Dombrowski cultivated milk yeast 

 in a medium of the following com- 

 position: 



1. Water 1000.0 cc. 



2. MgS04 (0.1%) 1.0 g. 



3. K2HPO4 (0.2%) 2.0 g. 



4. Dextrose (5.0%) 50.0 g. 



0. Agar (1.5%) 15.0 g. 



(b) Beijerinck cultivated Odium lactis 

 in a medium composed of the follow- 

 ing constituents: 



1. Water 1000.0 cc. 



2. Agar 20.0 g. 



