424 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



1443. Groenewege's Basal Sucrose Agar 



Constituents: 



1. Water (tap) 1000.0 cc. 



2. Agar to solidify 



3. Sucrose 20.0 g. 



4. K2HPO4 0.5 g. 



Preparation: (1) Dissolve 3, 4 and one of 



the added nutrients in tap water agar 



(preparation not given). 

 Sterilization: Not specified. 

 Use: Cultivation of Phytobacter lycoper- 



sicum n. sp. causing tomato rot. Author 



reported growth with all nitrogen sources 



employed. 

 Added nutrients: The author added one of 



the following nitrogen compounds as a 



nitrogen source: 



KNO3 



(NH4)2S04 



sodium ammonium tartrate 

 ammonium citrate 

 ammonium succinate 

 ammonium acetate 

 ammonium lactate 

 ammonium malate 

 asparagin 

 Reference: Groenewege (1913 p. 25). 



1444. Owen's Sucrose Agar 



Constituents: 



1. Water 1000.0 cc. 



2. Agar (2.0%) 20.0 g. 



3. Sucrose (Second 80°) 

 (10.0%) 100.0 g. 



Preparation: (1) Dissolve 2 and 3 in 1. 



Sterilization: Method not given. 



Use: To determine bacterial count in cane 



sugar products. 

 Reference: Owen (1914 p. 338). 



1445. Buchanan's Sucrose Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Agar 10.0 g. 



3. Sucrose 20.0 g. 



4. KH2PO4 0.2 g. 



5. MgS04 0.01 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Pour sterile (1) into sterile plates. 

 Sterilization: Method not specified. 



Use: To study the production of gum by 

 B. radicicola, isolated from a variety of 



hosts. Various authors used the same 

 or similar media for a variety of purposes. 

 Variants : 



(a) Peklo cultivated plant actinomyces 

 on a medium solidified with agar con- 

 taining 0.5% KH,P04, 0.2% MgS04 

 and did not specify the use of 1.0% 

 agar as did Buchanan. 



(b) Tanner, citing Moore, cultivated Ps. 

 radicicola on a medium containing 

 0.1% K2HPO4, 0.05% MgS04, 1.0% 

 sucrose and 1.0% agar. 



(c) Shunk dissolved 1.0 g. KH2PO4, 0.5 g. 

 MgS04, 10.0 g. sucrose and 10.0 g. or 

 15.0 g. agar in tap water. This me- 

 dium was employed for the isolation 

 of nodule bacteria. He reported that 

 if mannitol be substituted for sucrose, 

 the medium could be used to main- 

 tain cultures. 



References: Buchanan (1909 p. 381), Peklo 

 (1910 p. 470), Tanner (1919 p. 50), Shunk 

 (1921 p. 241). 



1446. Lohnis' Congo Red Sucrose Agar 



Bonstituents: 



1. Water 



2. Sucrose 



3. K2HPO4 



4. MgS04 



5. Agar 



6. Congo red 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 

 Sterilization: Not specified. 

 Use : Differentiation of B. tumefaciens and 



B. radicicola. Author reported that 



B. tumefaciens absorbed the congo red 



giving red colonies while B. radicicola 



formed white colonies. 

 Variants: (a) Giltner specified the use of 



washed agar. 

 References: Lohnis (1913 p. 112), Giltner 



(1921 p. 377). 



1447. Krainsky's Basal Starch Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Agar to solidify 



3. Starch (1.0%) 10.0 g. 



Preparation : 



(1) Dissolve 3 and one of the added nu- 

 trients in 1. 



