CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



425 



(2) Solidify (1) by the addition of agar. 

 Sterilization: Not given. 

 Use: Cultivation of Actinomyces. 

 Added nutrients: The author added 0.05% 

 of one of the following: 



KNO2 Peptone 



KXO3 Asparagin 



NH4CI 

 Variants: Noyes dissolved 1.0 or 2.0 g. of 

 starch and 15.0 g. agar in 1000.0 cc. of 

 water, and used the medium for soil 

 organisms. 

 References: Krainsky (1914 p. 662), Noyes 

 (1916 pp. 93, 94). 



1448. Wyant and Tweed's Starch Agar 



Constituents: 



1. Water..... 1000.0 cc. 



2. Starch (potato) 10.0 



3. Agar 10.0 g. 



4. K2HPO4 0.2 g. 



5. MgS04 0.2 g. 



6. K2CO3. 0.4 



7. CaCls 0.02 



8. Fe2(S04)3 0.02 _ 



9. NaCl 0.02 g. 



Preparation: 



(1) Prepare a starch solution bj^ suspend- 

 ing 10.0 g. potato starch in a little 

 cold water. Then add 500.0 cc. boil- 

 ing water. Concentrate to 500.0 cc. 



(2) Dissolve 3, 4, 5, 6, 7, 8 and 9 in 

 500.0 cc. water by boiling. 



(3) Replace amount of water lost by addi- 

 tion of distilled water. 



(4) Add (1) to (3). 

 Sterilization: Not specified. 



Use: Cultivation of organisms causing flat 



sour in peas. 

 Reference: Wyant and Tweed (1923 p. 12). 



1449. Welch's Dextrin Agar 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 

 Sterilization: Not specified. 

 Use: Isolation of amoeba. 



Reference: Welch (1917 p. 5) taken from 

 (1917 p. 396). 



1450. Hesse's Glycerol Agar 



Constituents: 



1. Distilled water 960.0 cc. 



2. Glycerol 30.0 g. 



3. Agar 10.0 g. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Filter and distribute in 20.0 cc. lots 

 in 50.0 cc. Jena glass flasks. 



(3) Melt the sterile agar and cool to 

 40 °C. 



(4) Place a portion of the sputum upon a 

 piece of litmus paper. 



(5) Add 1/10 N KOH to the agar until 

 the same shade of blue is given to 

 litmus paper as is given by the spu- 

 tum. One may take a series of 6 

 flasks containing 20.0 cc. of distilled 

 water and add 0.0, 0.2, 0.5, 1.0, 2.0 

 and 5.0 cc. respective of 1/10 N KOH 

 to the flasks and in this manner 

 determine the proper amount of KOH 

 to add to the agar to give the desired 

 reaction. 



(6) Pour the agar into sterile Petri 

 dishes. 



Sterilization: Sterilize for 3 hours in 

 streaming steam or autoclave for 1 hour 

 under two atmospheres of pressure. 



Use: Isolation of tubercle bacilli from 

 sputum. Author reported that the colo- 

 nies were seen under the microscope after 

 1 or 2 days. If accompanying bacteria 

 are to be studied add a small amount of 

 Nahrstoff Heyden or 0.1% grape sugar. 



References: Hesse (1904 p. 386), Abel (1912 

 p. 95). 



1451. Beijerinck's Mannitol Agar 



Same as medium 121, but solidified by the 

 addition of 2.0% agar. 



1452. Lohnis' Basal Glycerol Agar 



Constituents: 



1. Distilled water 1000.0 cc. 



2. K2HPO4 0.5 g. 



3. MgS04 0.02 g. 



4. NaCl 0.02 g. 



5. FejCU trace 



6. Agar (1.5%) 15.0 g. 



7. Glycerol (1.0%) 10.0 g. 



