426 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Sterilization : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Dissolve 1.0% glycerol and 0.1% of 

 one of the added nutrients to (1). 



(3) Distribute in 10.0 cc. quantities in 

 50.0 cc. Erlenmeyer flasks. 



Sterilization: Not specified. 



Use: To study assimilation of amide, am- 

 monia and nitrate. The author suggested 

 that any desired carbohydrate, alcohol, 

 etc., might replace the glycerol. 



Added nutrients: The author suggested the 

 addition of 0.1% of one of the following: 

 Urea Ammonium acetate 



Uric acid Ammonium butyrate 



Sodium hippurate Ammonium lactate 

 Asparagin NaNOj 



(NH 4)280 4 



Reference: L5hnis (1913 p. 99). 



1453. Sackett's Mannitol Salt Agar 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Na2S04 9.0668 g. 



3. NaCl 20.2621 g. 



4. NazCOs 2.0118 g. 



5. MgS04.... 1.7475 g. 



6. Mannitol 15.0 g. 



7. CaS04 9.4457 g. 



8. NaNOs 13.5319 g. 



9. Agar 15.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7 and 8 in 1. 



(2) Dissolve 15.0 g. of agar in 1000.0 cc. 

 of (1). 



(3) Tube. 



Sterilization: Autoclave at 120°C. for 5 

 minutes. 



Use: To study pigment production by 

 Azotobacter chroococcum. The author 

 reported generally a brownish pigment 

 was produced. No pigment was pro- 

 duced if mannitol be omitted. 



Variants: The author gave the following 

 variants : 



(a) Omitted the CaS04 and NaNO, and 

 added 2.8589 g. NajCOs. 



(b) Omitted the NaCl. 



(c) Omitted the NaNO 3. 



(d) Omitted the Na2S04. 



(e) Omitted the MgSOi. 



(f) Omitted the K2SO4. 



(g) Diluted any of the above media with 

 2 or 3 volumes of water. 



Reference: Sackett (1912 p. 109). 



1454. Ashby's Mannitol Agar (Jones) 



Same as medium 170, but solidified by 

 the addition of 1.5% agar. 



1455. Beijerinck's Mannitol Agar 

 (Omeliansky and Ssewerowa) 



Same as medium 148, but solidified by the 

 addition of 1.5% agar. 



Murray gave a modification of the same 

 medium by solidifying medium 149 by the 

 addition of 1.5% agar. 



1456. Shunk's Mannitol Agar 



Variant (c) of medium 1445 solidified 

 with agar. 



1457. Krainsky's Mannitol Agar 



Same as medium 188, but solidified by 

 the addition of 2.0% agar. 



1458. Heinemann's Mannitol Agar 

 Constituents: 



1. Distilled water 1000.0 cc. 



2. K2HPO4 0.2 g. 



3. CaCl2 0.02 g. 



4. MgS04 0.2 g. 



5. FejCl, 0.01 g. 



6. Mannitol 15.0 g. 



7. Agar 20,0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Add NaOH solution until the reac- 

 tion is neutral to phenolphthalein. 



(3) Dissolve 20.0 g. of agar in (2). 

 Sterilization: Sterilize in the autoclave. 

 Use: Cultivation of cultures capable of 



assimilating atmospheric nitrogen. 

 Reference: Heinemann (1922 p. 39). 



1459. Lleske's Acetate Agar 

 Constituents : 



1. Distilled water 1000.0 cc. 



2. Agar 10.0 g. 



3. Manganese acetate 0.1 g. 



Preparation: (1) Dissolve 2 and 3 in 1. 

 Sterilization: Not specified. 



Use: Isolation of Leptothrix ochracea. 

 Inoculate the agar plates when the sur- 

 face is still moist. 



Reference: Lieske (1919 p. 418). 



1460. Sohngen's Petroleum Agar 

 Constituents: 



1. Distilled water 1000.0 cc. 



2. Bipotassium phosphate 0.5 g. 



