CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



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3. MgSOi 0.5 g. 



4. Agar 20.0 g. 



5. Petroleum 

 Preparation : 



(1) Dissolve 2, 3 and 20.0 g. of washed 

 agar in 1. 



(2) Adjustment of reaction not given. 



(3) Pour in Petri dishes. 



(4) Invert the Petri dishes, and place a 

 little petroleum in a flat container, 

 on the lid inside the petri dish. This 

 places the surface of the medium 

 directly opposite the surface of the 

 petroleum. 



Sterilization: Method not given. 



Use: Cultivation of petroleum oxidizers, 

 soil forms Mycobacterium hyalinum 

 Mycobacterium phlei, Mycobacterium lac- 

 ticola, Mycobacterium rubrum, Mycobac- 

 terium album. 



Reference: Sohngen (1913 p. 59S). 



1461. Sohngen's Manganese Oxide Agar 



Constituents: 



1. Water agar 1000.0 cc. 



2. Glucose 5.0 g. 



3. K5HPO4 0.05 g. 



4. NH4CI 0.05 g. 



5. Manganese oxide 

 Preparation : 



(1) Dissolve 2, 3 and 4 in water agar 

 (composition not given). 



(2) Add manganese oxide until a black 

 color is formed. 



Sterilization: Method not given. 



Use: Cultivation of manganese bacteria. 

 Author reported that a clearing of the 

 medium near the colony indicated the 

 decomposition of the manganese oxide. 

 Acetobacter melanogenum, B. lactis 

 aerogenes, B. herbicola, B. aceti, B. ran- 

 cens and others decomposed manganese 

 oxide. 



Reference: Sohngen (1914 p. 554). 



1462. Conn and Breed's Glucose Ammonium 



Chloride Agar 



Constituents: 



1. Water 1000.0 cc. 



2. Agar 15.0 g. 



3. Glucose 10.0 g. 



4. KNO3 1.0 g. 



5. CaCl. 0.5 g. 



6. K2HPO4 0.5 g. 



7. NH4CI 2.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6 and 7 



in 1. 

 Sterilization: Not specified. 

 Use: Test for reduction of nitrate. 

 Reference: Conn and Breed (1919 p. 278). 



1463. Fulmer and Grimes' Sucrose Ammo- 

 nium Chloride Agar 



Constituents: 



1. Distilled water 1000.0 cc. 



2. NH4CI 1.88 g. 



3. K.,HP04 1.0 g. 



4. CaCh 1.0 g. 



5. Sucrose 50.0 g. 



6. Agar... 15.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Wash agar several times in distilled 

 water. 



(3) Add agar to (1), heat in autoclave at 

 15 pounds pressure for 30 minutes. 



(4) Filter thru absorbent cotton and tube. 

 Sterilization: Sterilize in live steam for 



30 minutes on two successive days. 

 Use: Cultivation of yeast, Saccharomyces 



cerevisiae and Torula sphaerica. 

 Variants: The authors omitted the CaCU- 



Other experiments were reported omitting 



the sucrose. 

 Reference: Fulmer and Grimes (1923 



p. 586). 



1464. Groenewege's Cellulose Agar 



Constituents: 



1. Water agar 100.0 cc. 



2. Cellulose 2.0 g. 



3. NH4CI 0.1 g. 



4. K2HPO4 0.05 g. 



Preparation: (1) Dissolve 2, 3 and 4 in tap 



water agar (composition not given). 



Sterilization: Not specified. 



Use: Cultivation of Phytobacter lycoper- 

 sicum causing tomato rot. Author re- 

 ported that organism did not utilize 

 cellulose. A slight growth appeared 

 after several weeks, however, due possibly 

 to a trace of starch in the filter paper used 

 as cellulose source. 



Reference: Groenewege (1913 p. 25). 



1465. Sohngen's Organic Acid Agar 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Agar 20.0 g. 



3. NH4CI 0.5 g. 



