428 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



4. MgS04... 0.5 g. 



5. Bipotassium phosphate 0.5 g. 



6. Sodium butyrate 10.0 g. 



7. Chemical elements 

 Preparation : 



(1) Dissolve 2, 3, 4 and 6 in 1. 



(2) Add several elements. (Elements or 

 compounds not given.) 



Sterilization: Not given. 



Use: Cultivation of Mycobacteria. Au- 

 thor reported that sulphur, potassium, 

 phosphorous nitrogen and carbon were 

 necessary for growth of mycobacteria. 

 The author also studied the production of 

 manganese oxide by bacteria on a similar 

 medium. 



Variants: The author reported that when 

 manganese oxide was produced on the 

 following medium a dark brown or black 

 colony was formed: 



1. Water agar 1000.0 cc. 



2. NH4CI 0.5 g. 



3. K2HPO4 0.5 g. 



4. Calcium malate or calcium 

 gluconate 20.0 g. 



5. Manganese sulphate 10.0 g. 



Reference: Sohngen (1913 p. 605), (1914 



p. 546). 



1466. Krainsky's Malate Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Agar to solidify 



3. NH4CI (0.05%). 0.5 g. 



4. Calcium malate (1.0%) 10.0 g. 



Preparation : 



(1) Dissolve 0.05% NH4CI and 1.0% 

 calcium malate (or the author sub- 

 stituted 1.0% calcium nitrate) in 

 water. 



(2) Solidify (1) by the addition of agar. 

 Sterilization: Method not given. 



Use: Cultivation of actinomyces. 

 Reference: Krainsky (1914 p. 662). 



1467. Noyes' Starch Ammonium Nitrate 

 Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Agar (best) 15.0 g. 



3. NH4NO3 1.0 g. 



4. Starch 1.0 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Method not given. 



Use: Cultivation of soil forms. 

 Reference: Noyes (1916 pp. 93, 94). 



1468. Kellerman and McBeth's Glucose 

 Ammonium Sulphate Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Agar 10.0 g. 



3. Glucose 10.0 g. 



4. K2HPO4 1.0 g. 



5. MgS04 1.0 g. 



6. NaCl 1.0 g. 



7. (NH4)2S04 2.0 g. 



8. CaCOa 2.0 g. 



Preparation : 



(1) Dissolve 4, 5, 6, 7 and 8 in 1000.0 cc. 

 of water. 



(2) Mix 500.0 cc. of (1) and 500.0 cc. of 

 water. 



(3) Dissolve 10.0 g. agar and 10.0 g. glu- 

 cose in (2). 



Sterilization: Not given. 



Use: Cultivation of Bacillus flavigena, 



Bacillus amylolyticus, Bacillus rossica 



n. sp. 

 Reference: Kellerman and McBeth (1912 



p. 487). 



1469. Higgins' Glucose Nitrate Agar 



Same as medium 270, but solidified by the 

 addition of agar. 



1470. Mortensen's Sucrose Ammonium 

 Sulphate Agar 



Same as medium 207, but solidified by 

 the addition of 2.0% agar. 



1471. Kellerman and McBeth's Polysac- 

 charide Ammonium Sulphate Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Cellulose 15.0 g. 



3. Agar 10.0 g. 



4. K2HPO4 1.0 g. 



5. MgS04 1.0 g. 



6. NaCl 1.0 g. 



7 (NH4)2S04 2.0 g. 



8. CaCOs 2.0 g. 



Preparation : 



(1) Prepare a liter of dilute ammonium 

 hydroxide solution by adding three 

 parts water to ten parts ammonium 

 hydroxide, sp. gr. 0.90. 



