430 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(3) Evaporate to 500.0 cc. 



(4) Dissolve 20.0 g. agar in 1000.0 cc. 

 water. 



(5) Dissolve 1.0 g. K2HPO4, 1.0 g. 

 MgSOi, 1.0 g. NaCl, 2.0g. (NH^z 

 SO4 and 2.0 g. CaCO, in (4). 



(6) Mix (3) and (5). 



(7) If deep agar tubes are to be pre- 

 pared it is well to filter until 

 clear. Filtering is carried out in 

 the autoclave. 



(8) Distribute in tubes and sterilize 

 in the autoclave at 100°C. for 

 15 minutes. 



(9) Medium may be poured into sterile 

 petri dishes. 



References: Kellerman and McBeth (1912 

 p. 487), Tanner (1919 pp. 61, 62), Giltner 

 (1921 p. 371), Khouvine (1923 pp. 714, 

 715). 



1472. Sanborn's Ammonium Sulphate 

 Cellulose Agar 

 Constituents: 



1. Distilled water 1000.0 cc. 



2. K2HPO4 10 g. 



3. MgS04 10 g. 



4. NasCO, 1.0 g. 



5. (NH4)2S04 2.0 g. 



6. Cotton 30.0 g. 



7. Agar 2.5 g. 



8. CR indicator (1.0%) 

 Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 500.0 cc. of 

 distilled water. 



(2) Prepare a 0.5% agar solution with 

 the remaining 500.0 cc. distilled 

 water. 



(3) Mix (1) and (2). 



(4) Cut 30.0 g. of cotton (chemically un- 

 treated) into small fragments. 



(5) Add 1.0% CR indicator with constant 

 stirring. (CR indicator prepared by 

 mixing equal parts of 0.5% aqueous 

 solution of china blue with 1.0% 

 solution of rosolic acid in 95.0% 

 alcohol.) 



(6) Mix well to insure an even distribu- 

 tion of cotton and pour into petri 

 dishes. 



Sterilization: The medium was autoclaved 

 after it had been added to the dishes. 

 The medium is colored red, pH-8.4. 



Use: To show fermentation of cellulose by 



Cellulomonas folia in the presence of Act. 

 colorata, Azotobacter, B. subtilis, B. 

 mycoids and B. cereus. 

 Reference: Sanborn (1926 p. 353). 



1473. Vierling's Cellulose Ammonium 

 Sulphate Agar 

 Constituents: 



1. Water 1000.0 cc 



2. K2HPO4 10 g. 



3. CaCl. 0.1 g. 



4. MgS04 0.1 g. 



5. FeCls trace 



6. NaCl trace 



7. Agar 20.0 g. 



8. Cellulose (Merck) 10.0 g. 



9. Potassium stearate 1.0 g. 



10. (NH4)2S04 5.0 g. 



11. CaCOs 10 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6, 7, 8, 



9, 10 and 11 in 1. 



Sterilization: Method not given. 



Use: Decomposition of cellulose by Myco- 

 bacteria. Incubate under a glass bell 

 jar. Author reported that the filter paper 

 showed no signs of being attacked. 

 Growth occurred, however. The agar 

 became turbid. After incubation no 

 clearing occurred around the colonies. 

 Cellulose was not decomposed. 



Reference: Vierling (1920 p. 206). 



1474. Proskauer and Beck's Glycerol Am- 

 monium Carbonate Agar (Klimmer) 



Constituents: 



1. Water 1000.0 cc. 



2. Glycerol 15.0 g. 



3. (NH4)2C03 3.5 g. 



4. MgS04 (crystalline) . . 2.5 g. 



5. KH2PO4 1-3 g. 



6. Agar 15.0 to 30.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 

 Sterilization: Method not given. 

 Use: Cultivation of tubercle bacilli. 

 Reference: Klimmer (1923 p. 172). 



1475. Bengis' Glucose Ammonium 

 Phosphate Agar 



Constituents : 



1. Distilled water 100.0 cc. 



2. Agar (powdered) 2.0 g. 



3. Glucose 2.0 g. 



4. (NH4)2HP04 0.1 g. 



