CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



431 



5. MgS04 0.5 g. 



6. CaCOa 1.0 g. 



Preparation : 



(1) Dissolve 2 in 1. 



(2) Add 3, 4, 5 and 6 to (1). 



(3) Filter to incubating flasks. 

 Sterilization: Sterilize in autoclave. 



Use: To cultivate large quantities of 



B. colt. 

 Reference: Bengis (1916 p. 392). 



1476. Ayers and Rupp's Fuchsin Sulphite 



Agar 

 Constituents: 

 1 Distilled water 1000.0 cc. 



2. Sodium ammonium phos- 

 phate 4.0 g. 



3. KH2PO4 2.0 g. 



4. Lactose 



5. Agar 30.0 g. 



6. Fuchsin (1.0% alcoholic 



basic soln.) 5.0 cc. 



7. NajSO, (5.0% aque. solu- 

 tion) 5.0 cc. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 500.0 cc. of 1. 



(2) Dissolve agar in 500.0 cc. distilled 

 water and filter. 



(3) Mix equal parts (1) and (2) and flask 

 in 100.0 cc. lots. 



(4) Prepare 1.0% alcoholic solution of 

 basic fuchsin. 



(5) Prepare 5.0% H2O solution of so- 

 dium sulfite. 



(6) To every 100.0 cc. of sterile (3) while 

 hottest, at time of plating add 0.5 cc. 

 of (4) and 0.5 cc. of (5) freshly pre- 

 pared. 



(7) Mix thoroughly. 



Sterilization: Method of sterilization of (3) 



not given. 

 Use: Enumeration of colon-aerogenes 

 group. Author reported the members of 

 the colon-aerogenes produced medium 

 sized red colonies with a ring around the 

 colony. Other colonies were pink. Puri- 

 fied litmus or brom cresol purple were not 

 as well suited as indicators as was the 

 fuchsin sulphite mixture. 

 Variants: Harvey prepared a similar me- 

 dium as follows: 



(1) Dissolve 2.0 g. sodium ammonium 

 phosphate, 1.0 g. KH2PO4, 5.0 g. lac- 

 tose, 15.0 g. agar in 1000.0 cc. distilled 

 water. 



(2) Add to this mixture at 90°C.: 1% 

 alcoholic basic fuchsin 0.5; freshly 

 prepared 5% sodium sulphite 0.5. 

 References: Ayers and Rupp (1918 pp. 433, 

 434), Harvey (1921-22 p. 103). 



1477. Cunningham's Cellulose Ammonium 

 Phosphate Agar 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Agar (1.5%) 15.0 g. 



3. Cellulose 



4. MgNH4P04 (0.05%) 0.5 g. 



5. KjHP04 (0.05%) 0.5 g. 



Preparation : 



(1) Wash 15.0 g. of agar by immersing it 

 in distilled water for about a week. 

 Change the water each day. 



(2) Add sufficient distilled water to make 

 a 1.5% agar solution. 



(3) Steam (2) for one hour. 



(4) Dissolve 0.05% Mg(NH4)P04 and 

 0.05% K2HPO4 in (3). 



(5) Tube in 4 to 8.0 cc. quantities. 



(6) Melt the sterile agar and pour an 

 8.0 cc. tube into a petri dish. 



(7) Place a sterile disc of filter paper on 

 the agar following inoculation and 

 gently press it down. Then pour 

 4.0 cc. of sterile melted agar over the 

 filter paper. 



Sterilization : Sterilize (5) intermittently in 

 steam. Sterilize, in the autoclave, pieces 

 of filter paper cut to fit a petri dish and 

 test tubes. 



Use: Decomposition of cellulose by soil 

 forms. 



Reference: Cunningham (1924 p. 140). 



1478. Bengis' Lactate Ammonium 

 Phosphate Agar 



Constituents : 



1. Distilled water 100.0 cc. 



2. (NH4)2HP04 0.2 g. 



3. Calcium lactate 1.0 g. 



4. Agar (powdered) 3.0 g. 



Preparation : 



(1) Dissolve 4 in 1. 



(2) Add and dissolve 2 and 3 in (1). 



(3) Filter into incubating flasks. 

 Sterilization: Sterilize in a Bramhall-Dean 



autoclave. 

 Use: To cultivate large quantities of 



B. coli. Author reported slight growth. 

 Reference: Bengis (1916 p. 392). 



