CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



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(NH4)2HP04 to (1) and heat for 

 10 minutes in a water bath. 



(3) Dissolve 10.0 g. of lactose in 2. 



(4) Filter. 



(5) Sterilize in the autoclave. 

 References: Dolt (1908 p. 625), Bengis 



(1916 p. 392). 



1482, Harrison and VanderLeck's Aesculin 

 Ammonium Phosphate Agar 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Agar 15.0 g. 



3. Glycerol 5.0 g. 



4. Ammonium phosphate 1.0 g. 



5. Iron citrate 2.5 g. 



6. Aesculin 1 .0 g. 



Preparation : 



(1) Purify agar by cutting it in small 

 pieces and soaking it in distilled 

 water for 24 hours. 



(2) Dis-solve (1), 3, 4 and 5 in 1. 



(3) Adjust reaction to +0.4. 

 Sterilization: Method not specified. 



Use: Detection of B. coli and B. typhosus. 



Authors reported that B. coli colonies 



were black, while typhoid colonies 



were not. 

 Reference: Harrison and VanderLeck (1909 



p. 622). 



1483. Bengis' Ammonium Lactate Agar 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Agar 25.0 g. 



3. NajHFOi 2.0 g. 



4. CaCO, 10.0 g. 



5. Ammonium lactate 10.0 g. 



Preparation: 



(1) Dissolve 2 and 3 in 1 in the autoclave. 



(2) Filter solution thru glass wool. 



(3) Adjust reaction to +0.3. 



(4) Distribute into liter Erlenmeyer 

 flasks in 500.0 cc. lots. The flasks 



containing 5.0 g. of CaCOs each. 



(5) Add 5.0 g. of ammonium lactate to 

 each flask. 



(6) Shake thoroly. 



Sterilization: Sterilize in autoclave at 



12 pounds pressure for 15 minutes. 

 Use: To cultivate large amounts of B. coli. 

 Variants : 



(a) The author omitted the CaCO 3. 



(b) Scales studied the variation in mor- 



phology of B. coli in the medium 

 using only 15.0 g. agar per liter. 

 References: Bengis (1916 p. 393), Scales 

 (1921 p. 595). 



1484. Cohn's Ammonium Tartrate Agar 

 (Klimmer) 



Constituents: 



1. Water 1000.0 cc. 



2. K2HPO4 5.0 g. 



3. MgS04 (Crystalline). 5.0 g. 



4. Calcium phosphate. . 0.5 g. 



5. Ammonium tartrate.. 10.0 g. 



6. Agar 15.0 to 30.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Solidify by dissolving 15.0 to 30.0 g. 

 of agar in (1). 



Sterilization: Not specified. 



Use: General synthetic culture medium. 



Reference: Klimmer (1923 p. 172). 



1485. Nelson's Ammonium Succinate Agar 



Constituents: 



1. Distilled water 1000.0 cc. 



2. K2HPO4 2.0 g. 



3. Ammonium succinate 2.0 g. 



4. NaCl 2.0 g. 



5. Glucose 2.0 g. 



6. Agar 20.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Reaction of pH = 7.0 to 7.2 (un- 

 adjusted). 



Sterilization: Sterilize at 10 pounds pres- 

 sure for 20 minutes. 



Use: Cultivation of colon-typhoid group 

 in mass culture. 



Reference: Nelson (1926 p. 373). 



1486. Dolt's Lactose Lactate Agar 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Agar (purified) 30.0 g. 



3. Ammonium lactate 5.0 g. 



4. Na2HP04 1.0 g. 



5. Lactose 10.0 g. 



6. Azolitmin (1.0% solution).. 10.0 cc. 

 Preparation : 



(1) Purify agar by cutting it into small 

 pieces and soaking in distilled water 

 24 hours. 



(2) Dissolve (1), 2, 3 and 4 in 1. 



