434 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(3) Make neutral to phenolphthalein with 

 NaOH. 



(4) Dissolve 5 in (3). 



(5) Prepare 6 by dissolving 1.0 g. Kahl- 

 baum's C. P. azolitmin in 100.0 cc. 

 distilled water. Boil 15 minutes. 

 The solution is blue. 



(6) Add (5) to (4). 

 Sterilization: Method not given. 



Use: Detection of acid production in 

 water analysis. Bengis used a similar 

 medium for the cultivation of B. coli in 

 large quantities. 



Variants : (a) Bengis omitted the azolitmin 

 solution, used 2.0 g. Na2HP04 instead of 

 1.0 g., and used 10.0 g. ammonium lactate 

 instead of 5.0 g. He reported that the 

 medium gave excellent results without 

 lactose. 



References: Dolt (1908 p. 626), Bengis (1916 

 p. 392). 



1487. Harrison and VanderLeck's Aesculin 

 Lactate Agar 



Constituents : 



1. Distilled water. 1000.0 cc. 



2. Agar 15.0 g. 



3. Ammonium lactate 5.0 g. 



4. Na2HP04 10 g- 



5. Iron citrate 2.5 g. 



6. Aesculin 1-0 g. 



Preparation: 



(1) Purify agar by cutting it in small 

 pieces and soaking them in water for 

 24 hours. 



(2) Dissolve (1), 3, 4, 5 and 6 in 1. 



(3) Adjust reaction to 0.4. 

 Sterilization: Not specified. 



Use: Detection of B. coli and B. typhosus. 

 Author reported that B. coli produced 

 black colonies, typhoid organisms did not. 



Reference : Harrison and VanderLeck (1909 

 p. 622). 



1488. Fischer's Nitrate Tartrate Agar 



Constituents : 



1. Water 1000.0 cc. 



2. Agar (1.25%) 12.5 g 



3. Dextrose (0.1%) 1-0 g 



4. Ammonium tartrate (0.1%). 1.0 g 



5. KNO3 (0.05%) 0.5 g 



6. Soda (crystalline) (0.15%). 1.5 g 



7. K2HPO4 (0.1%) 1.0 g 



8. CaCl2 (0.01%) 0.1 g 



9. MgS04 (Cr.) (0.03%) 0.3 g. 



10. NaCl (0.01%) 0.1 g. 



11. FeoCle (0.001%) 0.01 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6, 7, 8, 



9, 10 and 11 in 1. (7, 8, 9, 10 and 11 con- 

 stitute Meyers mineral solution in the 

 percentages given.) 



Sterilization: Method not given. 



Use: Bacterial count in soils. 



Reference: Fischer (1910 p. 457). 



1489. Sackett's Glucose Nitrate Agar 



Constituents : 



1. Water 1000 cc. 



2. Glucose 20.0 g. 



3. Agar 20.0 g. 



4. NaXOs 

 Preparation: 



(1) Dissolve 2 and 3 in 1. 



(2) Prepare a 10.0% NaNOs solution in 

 distilled water. 



(3) Add increasing amounts of (2) to (1) 

 so that the NaNOj concentration will 

 be 0.0, 0.01, 0.03, 0.05, 0.1, 0.3 and 

 0.5%. 



(4) Distribute in test tubes. 

 Sterilization: Sterilize in the autoclave for 



5 minutes at 120°C. 



Use: To study pigment production of 

 Azotobacter chroococcum. Author re- 

 ported that NaNOa favored the produc- 

 tion of a brown pigment, NH4CI, 

 (NH 4)280 4, asparagin and peptone did not 

 increase pigment production. 



Reference: Sackett (1913 p. 109). 



1490. Conn and Breed's Carbohydrate 

 Nitrate Agar 

 Constituents: 



1. Water 1000.0 cc. 



2. Agar 15.0 g. 



3. Glucose or sucrose 10.0 g. 



4. KNO3 10 g- 



5. CaCl.2 0.5 g. 



6. MgS04 5.0 g. 



7. K2HPO4 5.0 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6 and 7 



in 1. 

 Sterilization: Not specified. 

 Use: Study of reduction of nitrates. 

 Variants : 



(a) The authors omitted the MgS04 and 

 used only 0.5 g. K2HPO4 instead of 

 5.0 g. 



