CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



435 



(b) Hucker and Wall omitted the MgSOi 

 and used 0.5 g. K2HPO4 instead of 

 5.0 g. They used the medium to 

 determine ammonia production from 

 inorganic sources. Add 1.0 cc. of a 

 10.0% phenol and 1.0 cc. of a 1.0% 

 (available chlorine) sodium hypo- 

 chlorite solution to the surface of an 

 agar slant. A blue color forming 

 within 30 minutes denotes ammonia 

 production. Also 2.0 cc. of neutral 

 formaldehyde containing a few drops 

 of phenolphthalein may be added to 

 the slant. Presence of acid indicates 

 ammonia formation. 

 References: Conn and Breed (1919 p. 278), 

 Hucker and Wall (1922 p. 517), (1922 

 p. 485), Committee S. A. B. (1922 p. 525), 

 (1923 p. 27). 



1491. Groenewege's Sucrose Nitrate Agar 

 Constituents: 



1. Water agar 1000.0 cc. 



2. Sucrose 20 g 



3. KNO3 lOg" 



4. K2HPO, 0.5 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 



1000.0 cc. of tap water agar. (Composi- 

 tion not given.) 



Sterilization: Method not given. 



Use: Cultivation of Phytobacter lycoper- 

 sicum n. sp. (Causing tomato rot.) 



Reference: Groenewege (1913 p. 30). 



1492. Czapek's Sucrose Nitrate Agar (Conn) 

 Constituents: 



1- Water lOOO.O cc. 



2- Agar 15 ff. 



3. MgS04 



4. KCl 



5. K2HPO4 



6. FeS04 



7. NaNOa 



8. Sucrose 



Preparation: (1) Dissolve 2, 



7 and 8 in 1. 

 Sterilization: Method not specified. 

 Use: Cultivation of actinomyces. 

 Reference: Conn (1921 p. 21). 



1493. Pinoy's Dextrin Nitrate Agar 

 Constituents: 



1- Water 600.0 cc. 



2- Agar 9_0 g. 



3. Dextrin 15 g 



4. Cax\03 2 0g" 



5. KH2PO4 05 g 



6. KNO3 o.5g. 



7. MgS04 0.5 g. 



Preparation: 



(1) Prepare a 3.0% agar solution (9.0 g. 

 agar in 300.0 cc. of water). 



(2) Dissolve 3, 4, 5, 6 and 7 in 300.0 cc. 

 of water. 



(3) Mix equal parts sterile (1) and 

 sterile (2). 



Sterilization: Sterilize (1) and (2) sepa- 

 rately, method not given. 

 Use: Cultivation of B. fluorescens. 

 Reference: Pinoy (1907 p. 630). 



1494. Giltay's Glucose Nitrate Agar 

 (Giltner) 

 Constituents: 



1. Distilled water 1000 cc 



2. KH2PO4 2.0 g ' 



3. MgS04 20 g 



4. KNO3 10 g 



5. CaCl: 



0.2 g. 



10.0 g. 



6. FeoCIs soln 2 drops 



7. Agar (1.5%)., 15.O g. 



8. Citric acid 50 g. 



9. Glucose 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 8 in 800.0 cc. 

 distilled water. 



(2) Dissolve 1.5% of washed agar in (1) 

 by boiling. 



(3) Add a few drops of phenolphthalein. 



(4) Add sufficient 10.0% NaOH to turn 

 the solution a faint pink. 



(5) Filter thru absorbent cotton. 



(6) Dissolve 10.0 g. of glucose in (3). 



(7) Mix (5) and (6) thoroly and make up 

 to 1000.0 cc. 



Sterilization: Sterilize in the autoclave at 

 15 pounds pressure for 10 minutes. 



Use: To study denitrification. 



Variants: Heinemann used 20.0 g. agar in- 

 stead of 15.0 g. 



References: Giltner (1921 p. 376), Heine- 

 mann (1922 p. 40). 



1495. Conn and Breed's Double Sugar 

 Nitrate Agar 

 Constituents: 



1- Water 1000.0 cc. 



2. Agar 15.0 g. 



