436 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



3. Glucose. 



4. Lactose. 



5. KNO3... 



6. CaCh... 



7. K2HPO4 



10.0 g. 

 5.0 g. 

 1.0 g. 

 0.5 g. 

 0.5 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6 and 7 



in 1. 

 Sterilization: Method not specified. 

 Use: For reduction of nitrate. 

 Reference: Conn and Breed (1919 p. 278). 



SUBGROUP II-C. SECTION 2 



Basal or complete media containing agar 

 together with nitrogen and carbon of known 

 chemical composition. 

 Ai. Basal media used with the addition of 



other materials. 



Miehe's Basal Asparagin Agar. ..... 1496 



Fred and Peterson's Basal Asparagin 



Agar. 



1497 



Stuart's Basal Asparagin Agar 1498 



Long's Basal Glycerol Asparagin 



Agar (Uyei) 1499 



Aj. Complete media. 



Bi. Containing amino acids or their salts. 



Ci. Asparagin or sodium asparaginate 



employed. 

 Di. No additional organic carbon added. 



Noyes' Asparaginate Agar 1500 



Sullivan's Salt Asparagin Agar 1501 



Davis' Asparagin Phosphate Agar.. 1502 

 Bengis' Phosphate Asparagin Agar. 1503 

 Conn's Glucose Asparaginate Agar. 1504 

 Kisch's Glucose Asparagin Agar.. . . 1505 



Dolt's Lactose Asparagin Agar 1506 



D2. Additional organic carbon added. 



El. Only one type of organic carbon added. 



Fi. Carbohydrates added. 



Groenewege's Starch Asparagin 



Giltay's Citric Acid Asparagin Agar 



(Lohnis) • • • ■ 1^17 



El. More than one type of organic carbon 

 added. 



Conn's Asparaginate Agar 1518 



Smith's Levulose Asparaginate 



Agar. 



1507 



Agar 



Higgins' Basal Lactose Asparagm 



Agar. 



1519 



1520 



Noyes' Starch Asparaginate Agar . . 1508 

 Stutzer & Hartleb's Glycerol As- 

 paragin Agar, 1509 



Fj. Alcohols added. 



Sullivan's Glycerol Asparagin Agar. 1510 

 Peklo's Mannitol Asparagin Agar. . 1511 

 Thornton's Mannitol Asparagin 



Agar 1512 



Conn's Glycerol Asparaginate Agar. 1513 

 Fs. Organic acids or their salts added. 

 Beijerinck's Malate Asparagin Agar. 1514 

 Fraenkel's Lactate Asparagin Agar 



(Klimmer) 1515 



Voges' Lactate Asparaginate Agar.. 1516 



Sullivan's Glycerol-Nitrate Aspara- 

 gin Agar • • 1521 



Maassen's Malic Acid Asparagin 

 Agar (Klimmer) 1522 



Uschinsky's Glycerol Asparaginate 



Agar (Klimmer) 1523 



Cj. Other amino acids employed. 



Berthelot's Nitrate Tyrosein Agar.. 1524 



Jones' Histidin-hydro-chloride Agar. 1525 

 Bj. Not containing amino acids, or their 



salts. 



Sohngen's Malate Urea Agar 

 (Percival) ••• 1526 



Perotti's Glucose Dicyandiamide 

 Agar 1527 



Stapp's Uric Acid Agar 1528 



Stapp's Hippurate Agar 1529 



1496. Miehe's Basal Asparagin Agar 



Same as medium 379, but solidified by the 

 addition of agar. 



1497. Fred and Peterson's Basal Asparagin 

 Agar 



Constituents : 



1. Water '■ ■ ■ ■ 1000.0 cc. 



2. (NH4)2S04 3.0 g. 



3. Asparagin 1-5 g- 



4. K2HPO4 2.0 g. 



5. CaCU 0-25 g. 



6. MgS04 0.25 g. 



7. Agar 150 g. 



Preparation: 



(1) Dissolve 2, 3, 4, 5, 6, and 7 in 1. 



(2) Dissolve one of the sterile added 

 nutrients to sterile (1). 



(3) Reaction employed varied from pH- 

 6.5 to 7.2. 



Sterilization: Method not given. 



Use: To cultivate yeast found in sauer- 

 kraut. 



Added nutrients: Authors added 2.0% 

 glucose, maltose or xylose to the basic 

 agar medium. 



